Document Detail

n-3 PUFA fail to affect in vivo, antigen-driven CD8+T-cell proliferation in the spleen of naïve mice.
MedLine Citation:
PMID:  16571165     Owner:  NLM     Status:  MEDLINE    
One of the most frequently reported immunomodulatory actions of n-3 PUFA is their ability to diminish in vitro lymphocyte proliferation. The purpose of this study was to determine if n-3 PUFA intake affects the kinetics or magnitude of the antigen-driven expansion of CD8(+)T-lymphocytes in vivo. In this study we utilized a well-characterized model of T-cell immunity (i.e. infection with the intracellular bacterium, Listeria monocytogenes). Weanling BALB/c mice were fed one of two experimental diets that differed solely in fat source. Our control diet contained lard (180 g/kg) and was devoid of long-chain n-3 PUFA. The experimental diet contained 150 g/kg menhaden fish oil and 30 g/kg corn oil, thus providing approximately 8 % of energy from long-chain n-3 PUFA. After 4 weeks, mice were infected intravenously with 10(6) colony-forming units of actA-deficient L. monocytogenes. Clonal expansion of antigen-specific CD8(+)T-cells in the spleen was measured at 5, 7, 9 and 14 d post-challenge using a class I MHC tetramer loaded with the immunodominant peptide from this pathogen (i.e. K(d):LLO91-99). We report that feeding mice a diet rich in n-3 fatty acids did not significantly impact either the kinetics or magnitude of in vivo, antigen-driven expansion of CD8(+)T-cells. Furthermore, contraction of this T-cell population was not affected by n-3 PUFA treatment. To our knowledge this is the first time MHC tetramers have been used to investigate the influence of n-3 PUFA on in vivo CD8(+)T-cell proliferation.
Robert Irons; Kevin L Fritsche
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  The British journal of nutrition     Volume:  95     ISSN:  0007-1145     ISO Abbreviation:  Br. J. Nutr.     Publication Date:  2006 Apr 
Date Detail:
Created Date:  2006-03-30     Completed Date:  2006-06-05     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0372547     Medline TA:  Br J Nutr     Country:  England    
Other Details:
Languages:  eng     Pagination:  838-44     Citation Subset:  IM    
Nutritional Sciences Program, University of Missouri, Columbia, MO 65211, USA.
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MeSH Terms
Antigens, Bacterial / immunology
CD8-Positive T-Lymphocytes / drug effects*,  immunology
Cell Proliferation / drug effects
Cells, Cultured
Fatty Acids, Omega-3 / pharmacology*
Listeria Infections / immunology
Listeria monocytogenes / immunology
Lymphocyte Activation / drug effects*,  immunology
Mice, Inbred BALB C
Spleen / immunology*
Reg. No./Substance:
0/Antigens, Bacterial; 0/Fatty Acids, Omega-3

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