Document Detail


The myosin light chain enhancer and the skeletal actin promoter share a binding site for factors involved in muscle-specific gene expression.
MedLine Citation:
PMID:  2046675     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The myosin light chain (MLC) 1/3 enhancer (MLC enhancer), identified at the 3' end of the skeletal MLC1/3 locus, contains a sequence motif that is homologous to a protein-binding site of the skeletal muscle alpha-actin promoter. Gel shift, competition, and footprint assays demonstrated that a CArG motif in the MLC enhancer binds the proteins MAPF1 and MAPF2, previously identified as factors interacting with the muscle regulatory element of the skeletal alpha-actin promoter. Transient transfection assays with constructs containing the chloramphenicol acetyltransferase reporter gene demonstrated that a 115-bp subfragment of the MLC enhancer is able to exert promoter activity when provided with a silent nonmuscle TATA box. A point mutation at the MAPF1/2-binding site interferes with factor binding and abolishes the promoter activity of the 115-bp fragment. The observation that an oligonucleotide encompassing the MAPF1/2 site of the MLC enhancer alone cannot serve as a promoter element suggests that additional factor-binding sites are necessary for this function. The finding that MAPF1 and MAPF2 recognize similar sequence motifs in two muscle genes, simultaneously activated during muscle differentiation, implies that these factors may have a role in coordinating the activation of contractile protein gene expression during myogenesis.
Authors:
H Ernst; K Walsh; C A Harrison; N Rosenthal
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Molecular and cellular biology     Volume:  11     ISSN:  0270-7306     ISO Abbreviation:  Mol. Cell. Biol.     Publication Date:  1991 Jul 
Date Detail:
Created Date:  1991-07-17     Completed Date:  1991-07-17     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  8109087     Medline TA:  Mol Cell Biol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  3735-44     Citation Subset:  IM    
Affiliation:
Department of Medicine, Medical University of South Carolina, Charleston 29425.
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MeSH Terms
Descriptor/Qualifier:
Actins / genetics*
Animals
Base Sequence
Binding Sites
Cell Line
Chick Embryo
DNA-Binding Proteins / metabolism*
Enhancer Elements, Genetic*
Gene Expression Regulation*
Genetic Vectors
Methylation
Mice
Molecular Sequence Data
Muscles / physiology*
Myosins / genetics*
Nuclear Proteins / isolation & purification,  metabolism
Oligonucleotide Probes
Phosphoproteins / metabolism*
Promoter Regions, Genetic*
Transfection
Grant Support
ID/Acronym/Agency:
HD20686/HD/NICHD NIH HHS
Chemical
Reg. No./Substance:
0/Actins; 0/DNA-Binding Proteins; 0/Nuclear Proteins; 0/Oligonucleotide Probes; 0/Phosphoproteins; EC 3.6.4.1/Myosins
Comments/Corrections

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