Document Detail


myo-Inositol hexakisphosphate is a major component of an extracellular structure in the parasitic cestode Echinococcus granulosus.
MedLine Citation:
PMID:  11853537     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
myo-Inositol hexakisphosphate (IP(6)) is an abundant intracellular component of animal cells. In this study we describe the presence of extracellular IP(6) in the hydatid cyst wall (HCW) of the larval stage of the cestode parasite Echinococcus granulosus. The HCW comprises an inner cellular layer and an outer, acellular (laminated) layer up to 2 mm in thickness that protects the parasite from host immune cells. A compound, subsequently identified as IP(6), was detected in and purified from an HCW extract on the basis of its capacity to inhibit complement activation. The identification of the isolated compound was carried out by a combination of NMR, MS and TLC. The majority of IP(6) in the HCW was found in the acellular layer, with only a small fraction of the compound being extracted from cells. In the laminated layer, IP(6) was present in association with calcium, and accounted for up to 15% of the total dry mass of the HCW. IP(6) was not detected in any other structures or stages of the parasite. Our results imply that IP(6) is secreted by the larval stage of the parasite in a polarized fashion towards the interface with the host. This is the first report of the secretion of IP(6), and the possible implications beyond the biology of E. granulosus are discussed.
Authors:
Florencia Irigoín; Fernando Ferreira; Cecilia Fernández; Robert B Sim; Alvaro Díaz
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The Biochemical journal     Volume:  362     ISSN:  0264-6021     ISO Abbreviation:  Biochem. J.     Publication Date:  2002 Mar 
Date Detail:
Created Date:  2002-03-07     Completed Date:  2002-04-03     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  2984726R     Medline TA:  Biochem J     Country:  England    
Other Details:
Languages:  eng     Pagination:  297-304     Citation Subset:  IM    
Affiliation:
Cátedra de Inmunología, Facultad de Química/Ciencias, Universidad de la República, Avenida Alfredo Navarro 3051, piso 2, CP 11600, Montevideo, Uruguay.
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MeSH Terms
Descriptor/Qualifier:
Animals
Antigens, Helminth / analysis
Calcium / analysis
Cell Wall / chemistry
Chromatography, Thin Layer
Cyclophilin A / analysis
Echinococcus / chemistry*,  growth & development
Extracellular Space / chemistry
Helminth Proteins / analysis
Magnesium / analysis
Magnetic Resonance Spectroscopy
Mass Spectrometry
Phytic Acid / analysis*,  isolation & purification
Chemical
Reg. No./Substance:
0/Antigens, Helminth; 0/Helminth Proteins; 0/P-29 antigen; 7439-95-4/Magnesium; 7440-70-2/Calcium; 83-86-3/Phytic Acid; EC 5.2.1.-/Cyclophilin A
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