Document Detail

A mutation on influenza C virus M1 protein affects virion morphology by altering the membrane affinity of the protein.
MedLine Citation:
PMID:  17537859     Owner:  NLM     Status:  MEDLINE    
Reverse genetics has been documented for influenza A, B, and Thogoto viruses belonging to the family Orthomyxoviridae. We report here the reverse genetics of influenza C virus, another member of this family. The seven viral RNA (vRNA) segments of C/Ann Arbor/1/50 were expressed in 293T cells from cloned cDNAs, together with nine influenza C virus proteins. At 48 h posttransfection, the infectious titer of the culture supernatant was determined to be 2.51 x 10(3) 50% egg infectious doses/ml, which is lower than the number of influenza C virus-like particles (VLPs) (10(6)/ml) generated using the same system. By generating influenza C VLPs containing a given vRNA segment, we showed that each of the vRNA segments was similarly synthesized in the plasmid-transfected cells but that some segments were less efficiently incorporated into the VLPs. This finding leads us to speculate that the differences in incorporation efficiency into VLPs between segments might be a reason for the inefficient production of infectious viruses. Second, we generated a mutant recombinant virus, rMG96A, which possesses an Ala-->Thr mutation at residue 24 of the M1 protein, a substitution demonstrated to be involved in the morphology (filamentous or spherical) of the influenza C VLPs. As expected, rMG96A exhibited a spherical morphology, whereas recombinant wild-type of C/Ann Arbor/1/50, rWT, exhibited a mainly filamentous morphology. Membrane flotation analysis of the cells infected with rWT or rMG96A revealed a difference in the ratio of membrane-associated M1 proteins, suggesting that the affinity of M1 protein to the cell membrane is a determinant for virion morphology.
Yasushi Muraki; Toshio Murata; Emi Takashita; Yoko Matsuzaki; Kanetsu Sugawara; Seiji Hongo
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2007-05-30
Journal Detail:
Title:  Journal of virology     Volume:  81     ISSN:  0022-538X     ISO Abbreviation:  J. Virol.     Publication Date:  2007 Aug 
Date Detail:
Created Date:  2007-07-26     Completed Date:  2007-09-13     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  0113724     Medline TA:  J Virol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  8766-73     Citation Subset:  IM    
Department of Infectious Diseases, Yamagata University School of Medicine, Iida-Nishi, 990-9585, Yamagata, Japan.
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MeSH Terms
Alanine / chemistry
Amino Acid Sequence
Amino Acid Substitution
Cell Membrane / metabolism*
Cloning, Molecular
Influenzavirus C / genetics*,  growth & development*,  ultrastructure
Molecular Sequence Data
RNA, Viral / genetics,  metabolism
Threonine / chemistry
Viral Matrix Proteins / genetics,  metabolism*
Virion / genetics*,  growth & development*,  ultrastructure
Reg. No./Substance:
0/RNA, Viral; 0/Viral Matrix Proteins; 56-41-7/Alanine; 72-19-5/Threonine

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