| A mutation in beta-tubulin and a sustained dependence on androgen receptor signalling in a newly established docetaxel-resistant prostate cancer cell line. | |
| | |
MedLine Citation:
|
PMID: 19947927 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
The mechanisms of docetaxel resistance in PC (prostate cancer) are unclear because of the lack of suitable experimental models, and no effective treatment exists for docetaxel-resistant PC. We established a docetaxel-resistant cell line, LNDCr, from an androgen-refractory PC cell line, LNCaP-hr, by intermittent exposure to docetaxel in vitro. The LNDCr cells harboured an F270I mutation in class I beta-tubulin, and demonstrated impaired tubulin polymerization by docetaxel. AR signalling was sustained in LNDCr cells, and AR knockdown suppressed the growth of LNDCr cells. These results suggest that an acquired mutation in beta-tubulin is associated with docetaxel resistance in PC and that a novel AR-targeted therapy is effective for docetaxel-resistant PC. |
| | |
Authors:
|
Takahito Hara; Kazutaka Ushio; Mayumi Nishiwaki; Jin Kouno; Hideo Araki; Yukiko Hikichi; Masahiko Hattori; Yumi Imai; Masuo Yamaoka |
Related Documents
:
|
4335267 - Capillary tube scanning applied to cell growth kinetics. 6938517 - Comparative nuclear and cellular incorporation of daunorubicin, doxorubicin, carminomyc... 2528057 - Activity of thymidylate synthetase and its inhibition by 5-fluorouracil in highly enzym... 22678527 - Oleanolic acid arrests cell cycle and induces apoptosis via ros-mediated mitochondrial ... 22841977 - Calcium regulates the cell-to-cell water flow pathway in maize roots during variable wa... 19414367 - Effects of bortezomib on human neuroblastoma cells in vitro and in a metastatic xenogra... |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2010-01-25 |
Journal Detail:
|
Title: Cell biology international Volume: 34 ISSN: 1095-8355 ISO Abbreviation: Cell Biol. Int. Publication Date: 2010 Feb |
Date Detail:
|
Created Date: 2010-01-27 Completed Date: 2010-08-04 Revised Date: 2010-11-12 |
Medline Journal Info:
|
Nlm Unique ID: 9307129 Medline TA: Cell Biol Int Country: England |
Other Details:
|
Languages: eng Pagination: 177-84 Citation Subset: IM |
Affiliation:
|
Pharmacology Research Laboratories, Takeda Pharmaceutical Company Limited, Tsukuba, Japan. Hara_Takahito@takeda.co.jp |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Antineoplastic Agents
/
pharmacology* Cell Line, Tumor Drug Resistance, Neoplasm Humans Male Mutation Prostatic Neoplasms / metabolism* RNA Interference RNA, Small Interfering Receptors, Androgen / genetics, metabolism* Signal Transduction* Taxoids / pharmacology* Tubulin / genetics*, metabolism |
| Chemical | |
Reg. No./Substance:
|
0/AR protein, human; 0/Antineoplastic Agents; 0/RNA, Small Interfering; 0/Receptors, Androgen; 0/Taxoids; 0/Tubulin; 114977-28-5/docetaxel |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Cannabinoids modulate cell survival in embryoid bodies.
Next Document: The effect of N-acetylcysteine on chloride efflux from airway epithelial cells.