Document Detail

A mutation in the Schizosaccharomyces pombe rae1 gene causes defects in poly(A)+ RNA export and in the cytoskeleton.
MedLine Citation:
PMID:  7706287     Owner:  NLM     Status:  MEDLINE    
A collection of fission yeast Schizosaccharomyces pombe conditional mutants was screened for defective nucleocytoplasmic transport of poly(A)+ RNA by fluorescence in situ hybridization. We identified a temperature-sensitive mutant that accumulated poly(A)+ RNA in the nucleus and have named it rae1-1, for ribonucleic acid export. All rae1-1 cells exhibit the defect in poly(A)+ RNA export within 30 min following a shift to the non-permissive temperature. In addition, in the rae1-1 mutant, actin and tubulin become disorganized, and cells undergo an irreversible cycle arrest. Results from experiments in which rae1-1 cells were arrested in various phases of the cell division cycle and then shifted to nonpermissive temperature suggest that cells are particularly vulnerable to loss of rae1 function during G2/M. However, the inability to export RNA from the nucleus to the cytoplasm was not limited to a particular phase of the cell division cycle. The rae1 gene was isolated by complementation and encodes a predicted protein of 352 amino acids with four beta-transducin/WD40 repeats.
J A Brown; A Bharathi; A Ghosh; W Whalen; E Fitzgerald; R Dhar
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Publication Detail:
Type:  Comparative Study; Journal Article    
Journal Detail:
Title:  The Journal of biological chemistry     Volume:  270     ISSN:  0021-9258     ISO Abbreviation:  J. Biol. Chem.     Publication Date:  1995 Mar 
Date Detail:
Created Date:  1995-05-10     Completed Date:  1995-05-10     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  2985121R     Medline TA:  J Biol Chem     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  7411-9     Citation Subset:  IM    
Laboratory of Molecular Virology, NCI, National Institutes of Health, Bethesda, Maryland 20892, USA.
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MeSH Terms
Actins / analysis
Amino Acid Sequence
Base Sequence
Cell Cycle
Cell Division
Cell Nucleus / metabolism
Cloning, Molecular
Cytoplasm / metabolism
Cytoskeleton / metabolism*
DNA Primers
Fungal Proteins / biosynthesis*,  genetics
Genes, Fungal*
Genetic Complementation Test
In Situ Hybridization, Fluorescence
Molecular Sequence Data
Nuclear Matrix-Associated Proteins*
Nucleocytoplasmic Transport Proteins*
Polymerase Chain Reaction
RNA Processing, Post-Transcriptional / genetics*
RNA, Fungal / metabolism
RNA, Messenger / metabolism*
Restriction Mapping
Schizosaccharomyces / genetics*,  growth & development,  metabolism*
Schizosaccharomyces pombe Proteins*
Sequence Homology, Amino Acid
Tubulin / analysis
Reg. No./Substance:
0/Actins; 0/DNA Primers; 0/Fungal Proteins; 0/Nuclear Matrix-Associated Proteins; 0/Nucleocytoplasmic Transport Proteins; 0/RNA, Fungal; 0/RNA, Messenger; 0/Schizosaccharomyces pombe Proteins; 0/Tubulin; 0/rae1 protein, S pombe

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