Document Detail

A mutation defective in the xanthine alternative pathway of Aspergillus nidulans: its use to investigate the specificity of uaY mediated induction.
MedLine Citation:
PMID:  362158     Owner:  NLM     Status:  MEDLINE    
In Aspergillus nidulans uric acid can be produced from xanthine via purine hydroxylase I (xanthine dehydrogenase) or via the xanthine alternative pathway (Darlington and Scazzocchio, Biochem. Biophys. Acta, 166, 569--571; 1968). A mutation defective in the xanthine alternative pathway of Aspergillus nidulans is described. By combining this mutation with hxB-20 which results in complete loss of purine hydroxylase I and II activities, but which conserves cross-reacting material, it is possible to block completely uric acid production and thus investigate which are the effective in vivo inducers of three enzymes under the control of the positive regulatory gene uaY: adenine deaminase, purine hydroxylase I (measured as cross-reacting material) and urate oxidase. It is concluded that uric acid is the only effective physiological inducer, while its 2 and 8 thio-analogues serve as gratuitous inducers.
H M Sealy-Lewis; C Scazzocchio; S Lee
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Molecular & general genetics : MGG     Volume:  164     ISSN:  0026-8925     ISO Abbreviation:  Mol. Gen. Genet.     Publication Date:  1978 Sep 
Date Detail:
Created Date:  1979-01-24     Completed Date:  1979-01-24     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  0125036     Medline TA:  Mol Gen Genet     Country:  GERMANY, WEST    
Other Details:
Languages:  eng     Pagination:  303-8     Citation Subset:  IM    
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MeSH Terms
Aspergillus nidulans / genetics*
Enzyme Induction
Genes, Regulator*
Urate Oxidase / biosynthesis
Uric Acid / metabolism*
Xanthine Dehydrogenase / genetics
Xanthines / metabolism*
Reg. No./Substance:
0/Xanthines; 69-93-2/Uric Acid; EC Dehydrogenase; EC Oxidase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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