Document Detail


A mutant Drosophila homolog of mammalian Clock disrupts circadian rhythms and transcription of period and timeless.
MedLine Citation:
PMID:  9630223     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We report the identification, characterization, and cloning of a novel Drosophila circadian rhythm gene, dClock. The mutant, initially called Jrk, manifests dominant effects: heterozygous flies have a period alteration and half are arrhythmic, while homozygous flies are uniformly arrhythmic. Furthermore, these flies express low levels of the two clock proteins, PERIOD (PER) and TIMELESS (TIM), due to low per and tim transcription. Mapping and cloning of the Jrk gene indicates that it encodes the Drosophila homolog of mouse Clock. The mutant phenotype results from a premature stop codon that eliminates much of the putative activation domain of this bHLH-PAS transcription factor, thus explaining the dominant features of Jrk. The remarkable sequence conservation strongly supports common clock components present in the common ancestor of Drosophila and mammals.
Authors:
R Allada; N E White; W V So; J C Hall; M Rosbash
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Cell     Volume:  93     ISSN:  0092-8674     ISO Abbreviation:  Cell     Publication Date:  1998 May 
Date Detail:
Created Date:  1998-07-06     Completed Date:  1998-07-06     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  0413066     Medline TA:  Cell     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  791-804     Citation Subset:  IM    
Affiliation:
NSF, Center for Biological Timing, and Department of Biology, Brandeis University, Waltham, Massachusetts 02254, USA.
Data Bank Information
Bank Name/Acc. No.:
GENBANK/AF065133
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MeSH Terms
Descriptor/Qualifier:
Animals
Behavior, Animal
CLOCK Proteins
Chromosome Mapping
Circadian Rhythm / genetics*
Cloning, Molecular
Drosophila / genetics*
Drosophila Proteins*
Genes, Reporter
In Situ Hybridization
Insect Proteins / biosynthesis*,  genetics
Mammals / genetics
Molecular Sequence Data
Mutagenesis
Mutation
Nuclear Proteins / biosynthesis*
Period Circadian Proteins
Trans-Activators / genetics*
Transcription, Genetic
Chemical
Reg. No./Substance:
0/Drosophila Proteins; 0/Insect Proteins; 0/Nuclear Proteins; 0/PER protein, Drosophila; 0/Period Circadian Proteins; 0/Trans-Activators; 0/timeless protein, Drosophila; EC 2.3.1.48/CLOCK Proteins; EC 2.3.1.48/Clock protein, mouse

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