Document Detail


The murine myeloperoxidase gene contains a bipartite distal enhancer, including a novel region regulated by PEBP2/CBF.
MedLine Citation:
PMID:  8960105     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The myeloperoxidase (MPO) gene is expressed specifically in immature myeloid cells. DNA (27 kb) derived from the murine MPO gene was subcloned into nine segments, and each examined for its ability to enhance transcription in 32D cl3 myeloid cells. Only one segment, besides the known proximal enhancer, was active. Deletional analysis of this region delimited two functional regions. One is located 3.4 kb upstream of the proximal enhancer and was also recently identified in a different cell system. The second novel region is a 133 bp segment located 1.1 kb further upstream. This region, like the proximal enhancer, binds and is regulated by PEBP2/CBF. The PEBP2/CBF binding-site in the distal enhancer, AACCAAT, differs from the PEBP2/CBF consensus, PuACCPuCA.
Authors:
A D Friedman; M Britos-Bray; J Suzow
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Leukemia research     Volume:  20     ISSN:  0145-2126     ISO Abbreviation:  Leuk. Res.     Publication Date:  1996 Oct 
Date Detail:
Created Date:  1997-01-02     Completed Date:  1997-01-02     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  7706787     Medline TA:  Leuk Res     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  809-15     Citation Subset:  IM    
Affiliation:
Johns Hopkins Oncology Center, Division of Pediatric Oncology, Baltimore, MD 21287, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Cell Line
Cloning, Molecular
DNA / genetics
Enhancer Elements, Genetic* / genetics
Gene Expression Regulation, Enzymologic* / genetics
Granulocytes
Mice
Mice, Inbred BALB C
Peroxidase / biosynthesis,  genetics*
Transcription, Genetic* / genetics
Transfection
Grant Support
ID/Acronym/Agency:
HL51388/HL/NHLBI NIH HHS
Chemical
Reg. No./Substance:
9007-49-2/DNA; EC 1.11.1.7/Peroxidase
Comments/Corrections
Comment In:
Leuk Res. 1996 Oct;20(10):817-20   [PMID:  8960106 ]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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