| A murine model of bone marrow micrometastasis in breast cancer. | |
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MedLine Citation:
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PMID: 12498385 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Bone marrow (BM) is one of the most common sites and often the first clinical indication of metastatic progression of breast cancer. Multivariate analyses have shown that the presence of cytokeratin positive tumor cells in the marrow of women with newly diagnosed stage I, II or III breast cancer is an independent predictor of survival. The objective of this study was to develop an orthotopic model of spontaneous BM metastasis to facilitate studies of this process. A murine mammary adenocarcinoma cell line, Clone 66, was transduced with the neomycin resistance gene (Cl66neo) and injected orthotopically into female Balb/c mice. Polymerase chain reaction (PCR) for the neo gene performed on BM cells harvested from tumor bearing mice demonstrated as few as 10(2) injected tumor cells produced BM micrometastases at 4 weeks postinjection. Small foci of tumor cells were identified in the mammary fatpad (mfp) without gross evidence of primary tumors. Higher doses of tumor cells produced BM micrometastases, detectable by PCR, at one week post-injection. Constructs containing green fluorescent protein (GFP) and the neomycin resistance gene (neo) were also transduced into Clone 66 cells (Cl66-GFPneo) and injected into the mfp. GFP transduced tumor cells were identified in multiple tissues in addition to BM by flow cytometric analysis (FACS) but less 13% of the animals developed gross metastases. This model is a clinically relevant tool for the analysis of organ specificity of metastasis. |
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Authors:
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Barbara O'Kane Murphy; Shantaram Joshi; Anne Kessinger; Elizabeth Reed; J Graham Sharp |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Clinical & experimental metastasis Volume: 19 ISSN: 0262-0898 ISO Abbreviation: Clin. Exp. Metastasis Publication Date: 2002 |
Date Detail:
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Created Date: 2002-12-24 Completed Date: 2003-01-16 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 8409970 Medline TA: Clin Exp Metastasis Country: Netherlands |
Other Details:
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Languages: eng Pagination: 561-9 Citation Subset: IM |
Affiliation:
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Department of Genetics, Cell Biology and Anatomy, University of Nebraska Medical Center, Omaha, Nebraska 68198-6395, USA. bjmurphy@unmc.edu |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Adenocarcinoma
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genetics,
pathology*,
secondary* Animals Bone Marrow Neoplasms / pathology*, secondary* Breast Neoplasms / genetics, pathology* Disease Progression Female Flow Cytometry / methods Green Fluorescent Proteins Luminescent Proteins / analysis, genetics, metabolism Mice Mice, Inbred BALB C Neoplasms, Experimental / secretion Organ Specificity Polymerase Chain Reaction / methods Transduction, Genetic Tumor Cells, Cultured |
| Chemical | |
Reg. No./Substance:
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0/Luminescent Proteins; 147336-22-9/Green Fluorescent Proteins |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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