Document Detail


The murine gammaherpesvirus-68 chemokine-binding protein M3 inhibits experimental autoimmune encephalomyelitis.
MedLine Citation:
PMID:  20537410     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Chemokines are critical mediators of immune cell entry into the central nervous system (CNS), as occurs in neuroinflammatory disease such as multiple sclerosis. Chemokines are also implicated in the immune response to viral infections. Many viruses encode proteins that mimic or block chemokine actions, in order to evade host immune responses. The murine gammaherpesvirus-68 encodes a chemokine-binding protein called M3, which has unique biochemical features that enable it to bind to and inhibit an unusually broad range of chemokines. We applied a replication-defective adenoviral vector encoding M3 (AdM3) directly to the CNS to evaluate the capacity of this protein to inhibit neuroinflammation using the experimental autoimmune encephalomyelitis (EAE) model. Treatment with the AdM3 vector significantly reduced the clinical severity of EAE, attenuated CNS histopathology, and reduced numbers of immune cells infiltrating the CNS. These results suggest that M3 may represent a novel therapeutic approach to neuroinflammatory disease.
Authors:
Jason M Millward; Peter J Holst; Mette Høgh-Petersen; Allan R Thomsen; Jan P Christensen; Trevor Owens
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of neuroimmunology     Volume:  224     ISSN:  1872-8421     ISO Abbreviation:  J. Neuroimmunol.     Publication Date:  2010 Jul 
Date Detail:
Created Date:  2010-07-26     Completed Date:  2010-10-04     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8109498     Medline TA:  J Neuroimmunol     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  45-50     Citation Subset:  IM    
Copyright Information:
(c) 2010 Elsevier B.V. All rights reserved.
Affiliation:
Neuroimmunology Unit, Montreal Neurological Institute, 3801 University Street, Room 111, McGill University, Montreal, Quebec, Canada. jason.millward@charite.de
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MeSH Terms
Descriptor/Qualifier:
Adenoviridae / genetics
Animals
Cell Line
Cell Migration Inhibition / genetics,  immunology
Chemokines / antagonists & inhibitors,  metabolism*
Encephalomyelitis, Autoimmune, Experimental / genetics,  immunology*,  therapy*,  virology
Female
Gene Therapy / methods*
Gene Transfer Techniques*
Genetic Vectors / genetics,  therapeutic use
Humans
Mice
Mice, Inbred C57BL
Protein Binding / immunology
Rhadinovirus / genetics,  immunology*
Severity of Illness Index
Treatment Outcome
Viral Proteins / genetics,  therapeutic use*
Chemical
Reg. No./Substance:
0/Chemokines; 0/M3 protein, Murine gammaherpesvirus; 0/Viral Proteins

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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