| A murC gene from coryneform bacteria. | |
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MedLine Citation:
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PMID: 10091329 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The upstream flanking region of the ftsQ and ftsZ genes of Brevibacterium flavum MJ233, which belongs to the coryneform bacteria, was amplified by the inverse polymerase chain reaction method and cloned in Escherichia coli. Complementation analysis of E. coli mutant with a defective cell-wall synthesis mechanism with the cloned fragment and its DNA sequencing indicated the presence of the murC gene, encoding UDP-N-acetylmuramate:L-alanine ligase involved in peptidoglycan synthesis, just upstream from the ftsQ gene. The B. flavum murC gene could encode a protein of 486 amino acid residues with a calculated molecular mass of 51 198 Da. A 50-kDa protein was synthesized by the B. flavum murC gene in an in vitro transcription/translation system using E. coli S30 lysate. These results indicate that the genes responsible for cell-wall synthesis and cell division are located as a cluster in B. flavum similar to the E. coli mra region. |
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Authors:
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M Wachi; C D Wijayarathna; H Teraoka; K Nagai |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Applied microbiology and biotechnology Volume: 51 ISSN: 0175-7598 ISO Abbreviation: Appl. Microbiol. Biotechnol. Publication Date: 1999 Feb |
Date Detail:
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Created Date: 1999-04-30 Completed Date: 1999-04-30 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 8406612 Medline TA: Appl Microbiol Biotechnol Country: GERMANY |
Other Details:
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Languages: eng Pagination: 223-8 Citation Subset: IM |
Affiliation:
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Department of Bioengineering, Tokyo Institute of Technology, Yokohama, Japan. mwachi@bio.titech.ac.jp |
| Data Bank Information | |
Bank Name/Acc. No.:
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GENBANK/AB015023 |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Amino Acid Sequence Base Sequence Brevibacterium / enzymology, genetics* Cloning, Molecular DNA, Bacterial Escherichia coli / genetics, metabolism Genes, Bacterial* Genetic Complementation Test Molecular Sequence Data Mutation Peptide Synthases / chemistry, genetics*, metabolism* Peptidoglycan / biosynthesis |
| Chemical | |
Reg. No./Substance:
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0/DNA, Bacterial; 0/Peptidoglycan; EC 6.3.2.-/Peptide Synthases; EC 6.3.2.8/UDP-N-acetylmuramoyl-alanine synthetase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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