Document Detail


A multiplex nested PCR assay for simultaneous detection of Corchorus golden mosaic virus and a phytoplasma in white jute (Corchorus capsularis L.).
MedLine Citation:
PMID:  23413927     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A multiplex nested PCR assay was developed by optimizing reaction components and reaction cycling parameters for simultaneous detection of Corchorus golden mosaic virus (CoGMV) and a phytoplasma (Group 16Sr V-C) causing little leaf and bunchy top in white jute (Corchorus capsularis). Three sets of specific primers viz. a CoGMV specific (DNA-A region) primer, a 16S rDNA universal primer pair P1/P7 and nested primer pair R16F2n/R2 for phytoplasmas were used. The concentrations of the PCR components such as primers, MgCl2 , Taq DNA polymerase, dNTPs and PCR conditions including annealing temperature and amplification cycles were examined and optimized. Expected fragments of 1 kb (CoGMV), 674 bp (phytoplasma) and 370 bp (nested R16F2n/R2) were successfully amplified by this multiplex nested PCR system ensuring simultaneous, sensitive and specific detection of the phytoplasma and the virus. The multiplex nested PCR provides a sensitive, rapid and low-cost method for simultaneous detection of jute little leaf phytoplasma and CoGMV. Based on BLASTn analyses, the phytoplasma was found to belong to the Group 16Sr V-C.
Authors:
C Biswas; P Dey; S Satpathy
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2013-03-06
Journal Detail:
Title:  Letters in applied microbiology     Volume:  56     ISSN:  1472-765X     ISO Abbreviation:  Lett. Appl. Microbiol.     Publication Date:  2013 May 
Date Detail:
Created Date:  2013-04-12     Completed Date:  2013-07-05     Revised Date:  2013-11-04    
Medline Journal Info:
Nlm Unique ID:  8510094     Medline TA:  Lett Appl Microbiol     Country:  England    
Other Details:
Languages:  eng     Pagination:  373-8     Citation Subset:  IM    
Copyright Information:
© 2013 The Society for Applied Microbiology.
Affiliation:
Crop Protection Division, Central Research Institute for Jute and Allied Fibres (CRIJAF), Barrackpore, Kolkata, West Bengal, India. chinmaybiswas@rediffmail.com
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MeSH Terms
Descriptor/Qualifier:
Begomovirus / genetics,  isolation & purification*
Corchorus / genetics,  microbiology*,  virology
DNA Primers
DNA, Bacterial / genetics
DNA, Ribosomal / genetics
DNA, Viral / genetics
Mosaic Viruses / genetics,  isolation & purification
Multiplex Polymerase Chain Reaction / methods*
Phylogeny
Phytoplasma / classification,  genetics,  isolation & purification*
Plant Diseases / microbiology*,  virology*
Plant Leaves / microbiology,  virology
RNA, Ribosomal, 16S / genetics
Chemical
Reg. No./Substance:
0/DNA Primers; 0/DNA, Bacterial; 0/DNA, Ribosomal; 0/DNA, Viral; 0/RNA, Ribosomal, 16S

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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