Document Detail


A multipathogen selective enrichment broth for simultaneous growth of Salmonella enterica serovar Enteritidis, Staphylococcus aureus, and Listeria monocytogenes.
MedLine Citation:
PMID:  20651858     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A selective enrichment broth (SSL) was formulated to allow concurrent growth of 3 prominent food-borne pathogens: Salmonella enterica serovar Enteritidis, Staphylococcus aureus, and Listeria monocytogenes. Nalidixic acid, lithium chloride, and potassium tellurite were added as the selective agents, while sodium pyruvate and mannitol were employed as the supplemented elements. In the individual growth trial, the target pathogens were capable of growing in SSL to as high as 7-8 log(10) colony-forming units (CFU)/mL after 24 h incubation at 37 degrees C when being inoculated at 50-100 CFU/mL. In the simultaneous growth trial, the 3 combined target pathogens showed similar growth rates. The results show that SSL could support the successful simultaneous enrichment of 3 pathogens; however, SSL inhibited the growth of nontarget bacteria. In the artificial contaminated raw beef and ready-to-eat chicken, a high recovery of these 3 target pathogens was obtained in SSL. Finally, Salmonella Enteritidis, Staphylococcus aureus, and L. monocytogenes were detected from 710 suspicious food samples by SSL with real-time PCR, and no false-positive or -negative results were reported. In summary, SSL has been shown to be a suitable broth for the simultaneous detection of the 3 prominent food-borne pathogens by multipathogen detection on a single-assay platform.
Authors:
Yi-Gang Yu; Hui Wu; Yuan-Yuan Liu; Su-Long Li; Xiao-Quan Yang; Xing-Long Xiao
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Canadian journal of microbiology     Volume:  56     ISSN:  1480-3275     ISO Abbreviation:  Can. J. Microbiol.     Publication Date:  2010 Jul 
Date Detail:
Created Date:  2010-07-23     Completed Date:  2010-10-21     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0372707     Medline TA:  Can J Microbiol     Country:  Canada    
Other Details:
Languages:  eng     Pagination:  585-97     Citation Subset:  IM    
Affiliation:
Institution of Food Safety, South China University of Technology, Guangzhou, P.R. China.
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MeSH Terms
Descriptor/Qualifier:
Animals
Cattle
Chickens
Colony Count, Microbial
Culture Media / chemistry*,  metabolism
Food Contamination / analysis
Listeria monocytogenes / genetics,  growth & development*,  isolation & purification,  metabolism
Meat / microbiology
Salmonella enteritidis / genetics,  growth & development*,  isolation & purification,  metabolism
Staphylococcus aureus / genetics,  growth & development*,  isolation & purification,  metabolism
Chemical
Reg. No./Substance:
0/Culture Media

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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