Document Detail


A multiparametric PCR-based tool for fast detection and identification of spore-forming bacteria in food.
MedLine Citation:
PMID:  20637516     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The presence of psychrotrophic or highly thermoresistant spore-forming bacteria in food and feedstuff responsible for food poisoning and spoilage raises major safety and economical issues. The aim of this study was to evaluate the performances of a ready-to-use PCR assay (alternative method) in comparison with the standard microbiological plating method regarding spore-forming bacteria detection in food samples. An overnight sample enrichment was selected to increase sporeformer diversity recovery, spore germination, bacterial growth and favour DNA extraction. A total of 180 sporeformer isolates representing 38 different species and 8 genera were tested in the PCR assays. Inclusivity and exclusivity results ensured specific detection and identification of the majority of targeted genera and species. Validation studies carried on artificially contaminated food samples showed detection of the inoculated contaminants in most cases, with increased detection limit for the alternative method which enabled detection with up 1 spore of B. cereus in 25 g food sample. Using naturally contaminated food samples, standard method comforted the alternative method. In a number of cases, the alternative method was able to identify species not detected with the standard method. In addition, identification and discrimination between the B. cereus group members was possible. Thus, associated to a key element, i.e., the enrichment step, the developed multiparametric PCR-based assays reported in this study provide a fast, sensitive and reliable detection and identification tool for mostly encountered spore-forming food contaminants.
Authors:
Florence Postollec; Stéphane Bonilla; Florence Baron; Sophie Jan; Michel Gautier; Anne Gabrielle Mathot; Sylvie Hallier-Soulier; Sonia Pavan; Danièle Sohier
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Publication Detail:
Type:  Evaluation Studies; Journal Article; Research Support, Non-U.S. Gov't     Date:  2010-06-18
Journal Detail:
Title:  International journal of food microbiology     Volume:  142     ISSN:  1879-3460     ISO Abbreviation:  Int. J. Food Microbiol.     Publication Date:  2010 Aug 
Date Detail:
Created Date:  2010-08-10     Completed Date:  2010-11-22     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8412849     Medline TA:  Int J Food Microbiol     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  78-88     Citation Subset:  IM    
Copyright Information:
Copyright 2010 Elsevier B.V. All rights reserved.
Affiliation:
ADRIA Développement, UMT 08.3 PHYSI'Opt, Quimper, France. florence.postollec@adria.tm.fr
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MeSH Terms
Descriptor/Qualifier:
Bacteria / classification,  genetics,  isolation & purification*
Bacterial Typing Techniques / methods
DNA, Bacterial / genetics
Food Contamination / analysis
Food Microbiology*
Limit of Detection
Polymerase Chain Reaction / methods*
Spores, Bacterial / classification,  genetics,  isolation & purification*
Chemical
Reg. No./Substance:
0/DNA, Bacterial

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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