| A multinucleating Escherichia coli cytotoxin perturbs cell cycle in cultured epithelial cells. | |
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MedLine Citation:
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PMID: 17064876 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Pathogenic Escherichia coli strains produce a number of virulence-associated factors, among which cytotoxic necrotizing factor 1 (CNF1). CNF1 is a chromosomally encoded toxin that permanently activates the small GTP-binding proteins of the Rho family (Rho, Rac and Cdc42) by catalizing their deamidation at a specific glutamine residue. This activation modulates a high number of cellular functions, including the reorganization of the actin cytoskeleton, the promotion of cell spreading and the multinucleation. Indeed, accumulating evidence indicates that, in addition to the well-characterized Ras GTPases, also Rho family proteins are crucial in different points of cell cycle regulation. Here, we report that CNF1 induces a block of the cell cycle at the G(2)/M transition in epithelial cell line HEp-2, and up-regulates cyclin B1 and p53 proteins confining them in the cytoplasm region. The ability of CNF1 to perturb cell cycle progression could play a role in E. coli pathogenicity. |
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Authors:
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A Giamboi-Miraglia; S Travaglione; P Filippini; A Fabbri; C Fiorentini; L Falzano |
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Publication Detail:
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Type: Journal Article Date: 2006-09-14 |
Journal Detail:
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Title: Toxicology in vitro : an international journal published in association with BIBRA Volume: 21 ISSN: 1879-3177 ISO Abbreviation: Toxicol In Vitro Publication Date: 2007 Mar |
Date Detail:
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Created Date: 2007-02-12 Completed Date: 2009-07-21 Revised Date: 2009-11-19 |
Medline Journal Info:
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Nlm Unique ID: 8712158 Medline TA: Toxicol In Vitro Country: England |
Other Details:
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Languages: eng Pagination: 235-9 Citation Subset: IM |
Affiliation:
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Department of Drug Research and Evaluation, Istituto Superiore di Sanità, Rome, Italy. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Bacterial Toxins
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toxicity* Cell Cycle / drug effects* Cell Proliferation / drug effects Cells, Cultured Cyclin B / analysis Cyclin B1 Epithelial Cells / cytology, drug effects Escherichia coli Proteins / toxicity* Humans Tumor Suppressor Protein p53 / analysis |
| Chemical | |
Reg. No./Substance:
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0/Bacterial Toxins; 0/CCNB1 protein, human; 0/Cyclin B; 0/Cyclin B1; 0/Escherichia coli Proteins; 0/Tumor Suppressor Protein p53; 106803-33-2/cytotoxic necrotizing factor type 1 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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