Document Detail

A multinucleating Escherichia coli cytotoxin perturbs cell cycle in cultured epithelial cells.
MedLine Citation:
PMID:  17064876     Owner:  NLM     Status:  MEDLINE    
Pathogenic Escherichia coli strains produce a number of virulence-associated factors, among which cytotoxic necrotizing factor 1 (CNF1). CNF1 is a chromosomally encoded toxin that permanently activates the small GTP-binding proteins of the Rho family (Rho, Rac and Cdc42) by catalizing their deamidation at a specific glutamine residue. This activation modulates a high number of cellular functions, including the reorganization of the actin cytoskeleton, the promotion of cell spreading and the multinucleation. Indeed, accumulating evidence indicates that, in addition to the well-characterized Ras GTPases, also Rho family proteins are crucial in different points of cell cycle regulation. Here, we report that CNF1 induces a block of the cell cycle at the G(2)/M transition in epithelial cell line HEp-2, and up-regulates cyclin B1 and p53 proteins confining them in the cytoplasm region. The ability of CNF1 to perturb cell cycle progression could play a role in E. coli pathogenicity.
A Giamboi-Miraglia; S Travaglione; P Filippini; A Fabbri; C Fiorentini; L Falzano
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Publication Detail:
Type:  Journal Article     Date:  2006-09-14
Journal Detail:
Title:  Toxicology in vitro : an international journal published in association with BIBRA     Volume:  21     ISSN:  1879-3177     ISO Abbreviation:  Toxicol In Vitro     Publication Date:  2007 Mar 
Date Detail:
Created Date:  2007-02-12     Completed Date:  2009-07-21     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  8712158     Medline TA:  Toxicol In Vitro     Country:  England    
Other Details:
Languages:  eng     Pagination:  235-9     Citation Subset:  IM    
Department of Drug Research and Evaluation, Istituto Superiore di Sanità, Rome, Italy.
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MeSH Terms
Bacterial Toxins / toxicity*
Cell Cycle / drug effects*
Cell Proliferation / drug effects
Cells, Cultured
Cyclin B / analysis
Cyclin B1
Epithelial Cells / cytology,  drug effects
Escherichia coli Proteins / toxicity*
Tumor Suppressor Protein p53 / analysis
Reg. No./Substance:
0/Bacterial Toxins; 0/CCNB1 protein, human; 0/Cyclin B; 0/Cyclin B1; 0/Escherichia coli Proteins; 0/Tumor Suppressor Protein p53; 106803-33-2/cytotoxic necrotizing factor type 1

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