Document Detail


A multidisciplinary approach to describe protists: redescriptions of Novistrombidium testaceum Anigstein 1914 and Strombidium inclinatum Montagnes, Taylor, and Lynn 1990 (Ciliophora, Oligotrichia).
MedLine Citation:
PMID:  12836874     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We combined behavioral, morphological (live, stained, scanning and transmission electron-microscope), and molecular data to redescribe two common, intertidal oligotrich ciliates, Novistrombidium testaceum and Strombidium inclinatum. Both species were collected from a rocky shore near Leghorn, Ligurian Sea. A literature review revealed four morphotypes of Novistrombidium testaceum that differ in subtle ways, including oral morphology. These differences may be diagnostic, but we do not consider them sufficient to distinguish different taxa. Although other studies have synonymised Strombidium inclinatum and S. sulcatum, based on oral structures, size, and nuclear structure, there are morphological distinctions between them. In particular, the present study supports a lack of anterior protuberance in both live and preserved S. inclinatum, while S. sulcatum possesses a protuberance. The 18S rDNA molecular data, in accordance with morphological and ultrastructural observations, indicate that the Strombidiida (Oligotrichia) constitute a well-supported clade. The separation of the genera within this clade, even between Novistrombidium and Strombidium, remains unresolved, and the analysis of more species is required. Finally, we recommend that when possible, ecologists, morphological taxonomists, and molecular biologists combine their expertise to provide comprehensive taxonomic descriptions.
Authors:
Letizia Modeo; Giulio Petroni; Giovanna Rosati; David J S Montagnes
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  The Journal of eukaryotic microbiology     Volume:  50     ISSN:  1066-5234     ISO Abbreviation:  J. Eukaryot. Microbiol.     Publication Date:    2003 May-Jun
Date Detail:
Created Date:  2003-07-02     Completed Date:  2003-08-25     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  9306405     Medline TA:  J Eukaryot Microbiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  175-89     Citation Subset:  IM    
Affiliation:
Dipartimento di Etologia, Ecologia ed Evoluzione, Università di Pisa, Pisa 56126, Italy. modeo@deee.unipi.it
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MeSH Terms
Descriptor/Qualifier:
Animals
Base Sequence
Ciliophora / classification*,  isolation & purification,  ultrastructure
Eukaryota / isolation & purification
Microscopy, Electron, Scanning
Phylogeny
Polymerase Chain Reaction / methods
RNA, Ribosomal, 18S / chemistry
Seawater
Chemical
Reg. No./Substance:
0/RNA, Ribosomal, 18S

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