| A multicomponent mobile phase for ion chromatography applied to the separation of anions from the residue of low explosives | |
| | |
MedLine Citation:
|
PMID: 10845378 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
|
A multicomponent mobile phase utilizing ion-exchange, ion-exclusion, and ion-pairing principles for the rapid isocratic separation of anions in low explosives residue by ion chromatography (IC) has been developed. The notable feature of this system is that an ion-pairing reagent and an ion-exclusion reagent are combined in the same mobile phase. Contrary to expectation, these reagents act independently of each other in solution. The stock mobile-phase composition consisted of boric acid, D-gluconic acid, lithium hydroxide, and glycerol. Tetrapropylammonium hydroxide, an ion-interaction reagent was used to achieve pH 8.5. Acetonitrile (ACN) was added to enhance resolution and octanesulfonic acid, an ion-exclusion reagent, was added to adjust the retention time of perchlorate. Separation of a mixture of anions common to low explosives residue was achieved in less than 16 min using a Waters IC-Pak Anion HR column. Optimization studies were performed by changing the concentration of the ACN and by altering the pH or the type of ion-interaction or -exclusion agents. Simulated case studies were performed using postblast residues from pipe bombs. The results show this method to be a valid and reproducible procedure for forensic casework analysis. The practical significance of this system is that a reduction in the analysis time and an improvement in efficiency of late-eluting peaks can be achieved without resorting to gradient elution techniques. For the analysis of anions detected in explosives residue, the Waters IC-Pak Anion HR column has proven to be a suitable replacement for the Vydac 300IC405 column, which has been discontinued by the manufacturer. |
| | |
Authors:
|
Doyle; Miller; McCord; McCollam; Mushrush |
Related Documents
:
|
12214698 - Single column approach for the liquid chromatographic separation of polar and non-polar... 10768498 - Separation of purines and pyrimidines by normal-phase high-performance liquid chromatog... 23303748 - Characterization of superoxide dismutase 1 (sod-1) by electrospray ionization-ion mobil... 23430178 - Resonance-enhanced multiphoton ionization with circularly polarized light: chiral carbo... 22533908 - Development and comparison of three liquid chromatography-atmospheric pressure chemical... 16756368 - Chemical composition, seasonal variability, and antifungal activity of lavandula stoech... |
Publication Detail:
|
Type: JOURNAL ARTICLE |
Journal Detail:
|
Title: Analytical chemistry Volume: 72 ISSN: 1520-6882 ISO Abbreviation: Anal. Chem. Publication Date: 2000 May |
Date Detail:
|
Created Date: 2000-06-07 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 0370536 Medline TA: Anal Chem Country: UNITED STATES |
Other Details:
|
Languages: Eng Pagination: 2302-7 Citation Subset: - |
Affiliation:
|
Forensic Science Research and Training Center, FBI Laboratory, Quantico, Virginia 22135, USA. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Electroosmotic and pressure-driven flow in open and packed capillaries: velocity distributions and f...
Next Document: Microextraction of nine haloacetic acids in drinking water at microgram per liter levels with electr...