Document Detail


A multi-purpose microfluidic perfusion system with combinatorial choice of inputs, mixtures, gradient patterns, and flow rates.
MedLine Citation:
PMID:  19156291     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Microfluidic perfusion systems, characterized by deterministic flow, low reagent consumption, small dead volumes, large integration in small footprints, high-throughput operation, and low-cost fabrication, are being increasingly used for cell culture studies in applications such as basic cell biology, molecular biological assays, tissue engineering, and systems biology. We report a multipurpose, pressure-driven and computer-controlled microfluidic perfusion device containing sixteen inlets and a large cell culture chamber. The user can choose, with sub-second temporal resolution, (a) to feed the chamber with one of 16 inlets, all 16 inlets, or one of 64 combinations of 2, 4, or 8 inlets using a binary multiplexer; (b) to introduce into the chamber a heterogeneous laminar flow of the inlets, a smoothened gradient, or a fully homogenized mixture; (c) to bypass the chamber in order to purge the inlet lines so as to minimize the dead volume; (d) to generate asymmetrical and curvilinear flow patterns within the chamber by opening side outlets; and (e) to slow down the flow by combinatorially adding segments of high fluid resistance (sixteen different levels of flow rates are possible using only four valves). All functionalities are combined to create complex gradient patterns and sequential perfusions within the central chamber.
Authors:
Gregory A Cooksey; Christopher G Sip; Albert Folch
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural     Date:  2008-11-07
Journal Detail:
Title:  Lab on a chip     Volume:  9     ISSN:  1473-0197     ISO Abbreviation:  Lab Chip     Publication Date:  2009 Feb 
Date Detail:
Created Date:  2009-01-21     Completed Date:  2009-05-14     Revised Date:  2014-09-21    
Medline Journal Info:
Nlm Unique ID:  101128948     Medline TA:  Lab Chip     Country:  England    
Other Details:
Languages:  eng     Pagination:  417-26     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Algorithms
Cell Culture Techniques* / instrumentation
Dimethylpolysiloxanes / chemistry
Equipment Design
Microfluidic Analytical Techniques / instrumentation*
Perfusion / instrumentation*
Physical Processes
Grant Support
ID/Acronym/Agency:
DC006564/DC/NIDCD NIH HHS; EB003307/EB/NIBIB NIH HHS; R21 DC006465/DC/NIDCD NIH HHS; R21 DC006465-02/DC/NIDCD NIH HHS; R21 EB003307/EB/NIBIB NIH HHS; R21 EB003307-02/EB/NIBIB NIH HHS
Chemical
Reg. No./Substance:
0/Dimethylpolysiloxanes; 63148-62-9/baysilon
Comments/Corrections

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