Document Detail


A multi-omic map of the lipid-producing yeast Rhodosporidium toruloides.
MedLine Citation:
PMID:  23047670     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Triacylglycerols are among the most attractive alternative raw materials for biofuel development. Current oil plant-based technologies are limited in terms of triacylglycerol production capacity and rate. These limitations may be circumvented by biotransformation of carbohydrates into lipids; however, our understanding of microbial oleaginicity remains limited. Here we present the results of a multi-omic analysis of Rhodosporidium toruloides, a robust triacylglycerol-producing fungus. The assembly of genome and transcriptome sequencing data reveals a genome of 20.2 Mb containing 8,171 protein-coding genes, the majority of which have multiple introns. Genes including a novel fatty acid synthase are predicted to participate in metabolic pathways absent in non-oleaginous yeasts. Transcriptomic and proteomic data suggest that lipid accumulation under nitrogen-limited conditions correlates with the induction of lipogenesis, nitrogenous compound recycling, macromolecule metabolism and autophagy. The multi-omic map of R. toruloides therefore provides a valuable resource for efforts to rationally engineer lipid-production pathways.
Authors:
Zhiwei Zhu; Sufang Zhang; Hongwei Liu; Hongwei Shen; Xinping Lin; Fan Yang; Yongjin J Zhou; Guojie Jin; Mingliang Ye; Hanfa Zou; Hanfan Zou; Zongbao K Zhao
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Nature communications     Volume:  3     ISSN:  2041-1723     ISO Abbreviation:  Nat Commun     Publication Date:  2012  
Date Detail:
Created Date:  2012-10-10     Completed Date:  2013-02-01     Revised Date:  2013-09-26    
Medline Journal Info:
Nlm Unique ID:  101528555     Medline TA:  Nat Commun     Country:  England    
Other Details:
Languages:  eng     Pagination:  1112     Citation Subset:  IM    
Affiliation:
Division of Biotechnology, Dalian Institute of Chemical Physics, CAS, 457 Zhongshan Road, Dalian 116023, China.
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MeSH Terms
Descriptor/Qualifier:
Basidiomycota / genetics*,  metabolism*
Gene Expression Profiling / methods*
Genomics / methods*
Lipids / biosynthesis*
Proteomics / methods*
Chemical
Reg. No./Substance:
0/Lipids
Comments/Corrections
Erratum In:
Nat Commun. 2013;4:1930
Note: Zou, Hanfan [corrected to Zou, Hanfa]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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