| The Movat pentachrome stain as a means of identifying microcrystalline cellulose among other particulates found in lung tissue. | |
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MedLine Citation:
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PMID: 21284446 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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CONTEXT: Microcrystalline cellulose (MCC) is extensively used as a filler material in pharmaceutical tablets. When injected intravenously in aqueous tablet suspensions, MCC may contribute to embolic pulmonary hypertension and be identified histologically in lung tissue samples. In this study, we evaluated a modified Russell Movat pentachrome stain (MMPS) as a means of recognizing MCC and distinguishing it from other birefringent crystals in lung specimens. OBJECTIVE: To study the staining properties of MCC versus other crystalline materials using the MMPS stain. DESIGN: Archival, formalin-fixed, paraffin-embedded lung specimens that contained birefringent crystals, including MCC (3 cases of intravenous drug abuse), talc (2 cases of intravenous drug abuse, 1 talc pleurodesis), mixed silicates (1 case of silicate pneumoconiosis), and calcium oxalate (1 case of aspergillosis from Aspergillus niger infection), were evaluated with MMPS. Crystal identification was confirmed by morphology, other histochemical stains, infrared spectroscopy (1 case), and cellulose controls. RESULTS: The MMPS stained the MCC bright yellow in tissue and control specimens. Talc stained light greenish-blue; mixed silicates appeared either greenish-blue or unstained. Oxalate crystals stained sea-green. Crospovidone, a nonbirefringent tablet filler substance, stained yellow to dark green with MMPS and was easily distinguished from MCC. Starch granules were unstained by MMPS. CONCLUSIONS: The MMPS is an excellent method for the histochemical identification of MCC in tissue and its separation from other birefringent crystals with which MCC might be confused. The MMPS is especially useful in the evaluation of pulmonary foreign body embolization in cases of suspected intravenous substance abuse. |
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Authors:
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Saroj Sigdel; J Todd Gemind; Joseph F Tomashefski |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Archives of pathology & laboratory medicine Volume: 135 ISSN: 1543-2165 ISO Abbreviation: Arch. Pathol. Lab. Med. Publication Date: 2011 Feb |
Date Detail:
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Created Date: 2011-02-02 Completed Date: 2011-03-14 Revised Date: 2011-09-16 |
Medline Journal Info:
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Nlm Unique ID: 7607091 Medline TA: Arch Pathol Lab Med Country: United States |
Other Details:
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Languages: eng Pagination: 249-54 Citation Subset: AIM; IM |
Affiliation:
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Department of Pathology, MetroHealth Medical Center, Case Western Reserve University School of Medicine, Cleveland, Ohio, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Adult Aged Cellulose / analysis* Coloring Agents / diagnostic use* Female Humans Lung / pathology* Male Middle Aged Particulate Matter / analysis* Staining and Labeling / methods* Suspensions / adverse effects Tablets / adverse effects |
| Chemical | |
Reg. No./Substance:
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0/Coloring Agents; 0/Particulate Matter; 0/Suspensions; 0/Tablets; 0/microcrystalline cellulose; 9004-34-6/Cellulose |
| Comments/Corrections | |
Comment In:
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Arch Pathol Lab Med. 2011 Aug;135(8):963; author reply 963-4
[PMID:
21809983
]
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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