| A mouse with a monoclonal primary immunoglobulin repertoire not further diversified by V-gene replacement. | |
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MedLine Citation:
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PMID: 10636478 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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We have generated a monoclonal B-cell mouse by introducing homozygous, nonfunctional RAG-2 alleles and a lambda1 light-chain transgene into the quasi-monoclonal (QM) mouse, which contains a "knocked-in" V(H)DJ(H) rearrangement. Thus, this mouse, which we call MonoB, is devoid of T cells and contains preformed heavy- and light-chain genes encoding immunoglobulin with an anti-NP specificity. The MonoB mouse allows us to examine immunoglobulin diversity in the absence of processes mediated by V(D)J recombination and T cells. Here we report that not only is the MonoB's primary immunoglobulin repertoire monoclonal, but also that its secondary repertoire is not further diversified by V-gene replacement or gene conversion. Among 99 heavy-chain and 41 lambda light-chain genes from peripheral B cells of the MonoB mouse, there were no V-gene replacements. When compared to the QM mouse, which has RAG activity, and for which V-gene replacement is the major diversifying mechanism, these data suggest that V-gene replacement is mediated by V(D)J recombination and not by other recombination systems. |
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Authors:
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M Cascalho; D A Martin; J Wong; Q Lam; M Wabl; G E Wu |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Developmental immunology Volume: 7 ISSN: 1044-6672 ISO Abbreviation: Dev. Immunol. Publication Date: 1999 |
Date Detail:
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Created Date: 2000-02-11 Completed Date: 2000-02-11 Revised Date: 2008-11-21 |
Medline Journal Info:
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Nlm Unique ID: 9200624 Medline TA: Dev Immunol Country: ENGLAND |
Other Details:
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Languages: eng Pagination: 43-50 Citation Subset: IM |
Affiliation:
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Department of Microbiology and Immunology, University of California, San Francisco 94143, USA. |
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals B-Lymphocytes / immunology* Gene Rearrangement, B-Lymphocyte* Genetic Variation Immunoglobulin Isotypes / genetics*, immunology* Immunoglobulin Variable Region / genetics* Mice Mice, Knockout Mice, Transgenic |
| Grant Support | |
ID/Acronym/Agency:
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R01 AI41570/AI/NIAID NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Immunoglobulin Isotypes; 0/Immunoglobulin Variable Region |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
| Full Text | |
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Journal Information Journal ID (nlm-ta): Dev Immunol ISSN: 1044-6672 Publisher: Hindawi Publishing Corporation |
Article Information Download PDF  Copyright ? 1999 Hindawi Publishing Corporation. open-access: This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Accepted Day: 9 Month: 12 Year: 1998 Revision Received Day: 1 Month: 12 Year: 1998 Print publication date: Year: 1999 Volume: 7 Issue: 1 First Page: 43 Last Page: 50 ID: 2276038 PubMed Id: 10636478 Publisher Item Identifier: S1740252299245143 DOI: 10.1155/1999/24514 |
| A Mouse with a Monoclonal Primary lmmunoglobulin Repertoire not Further Diversified by V-Gene Replacement | |
| Marilia Cascalho1 | |
| Denise A. Martin2 | |
| Jamie Wong1 | |
| Queenie Lam2 | |
| Matthias Wabl1 | |
| Gillian E. Wu23 |
Address: gillian.wu@utoronto.ca |
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1Department of Microbiology and ImmunologyUniversity of CaliforniaSan FranciscoCA94143USA |
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2Department of ImmunologyOntario Cancer InstituteUniversity of TorontoTorontoOntarioM5G 2M9Canada |
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3Ontario Cancer InstituteRoom 8-113610 University AvenueTorontoOntarioM5G 2M9Canada |
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Article Categories:
Keywords: MonoB mouse, QM mouse, RAG, transgenic, knockin, flow cytometry. |
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