| The mouse tyrosinase promoter is sufficient for expression in melanocytes and in the pigmented epithelium of the retina. | |
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MedLine Citation:
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PMID: 1902569 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The mouse c locus encodes tyrosinase (monophenol monooxygenase; monophenol, L-dopa:oxygen oxidoreductase, EC 1.14.18.1), the key enzyme in melanin synthesis, which is expressed in the pigment epithelium of the retina and in melanocytes derived from the neural crest. To define regulatory regions of the gene that are important for cell type-specific expression, a deletion series of the tyrosinase 5' region was fused to a chloramphenicol acetyltransferase (CAT) reporter gene and electroporated into tyrosinase-expressing and -nonexpressing cell lines. We show that 270 base pairs 5' of the transcriptional start site is sufficient for CAT expression in a human and a mouse melanoma cell line. This 5' flanking fragment, when cloned in the context of a tyrosinase minigene construct and injected into fertilized eggs of an albino mouse strain, is sufficient for cell type-specific expression in mice. The transgenic mice were pigmented in both skin and eyes. In situ hybridization analysis shows that the 270-base-pair regulatory region contains elements sufficient for specific expression of the transgene both in the pigmented epithelial cells of the retina, which are derived from the optic cup, and in neural crest-derived melanocytes. |
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Authors:
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M Klüppel; F Beermann; S Ruppert; E Schmid; E Hummler; G Schütz |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Proceedings of the National Academy of Sciences of the United States of America Volume: 88 ISSN: 0027-8424 ISO Abbreviation: Proc. Natl. Acad. Sci. U.S.A. Publication Date: 1991 May |
Date Detail:
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Created Date: 1991-05-31 Completed Date: 1991-05-31 Revised Date: 2009-11-18 |
Medline Journal Info:
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Nlm Unique ID: 7505876 Medline TA: Proc Natl Acad Sci U S A Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 3777-81 Citation Subset: IM |
Affiliation:
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Institute of Cell and Tumor Biology, German Cancer Research Center, Heidelberg. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cloning, Molecular Gene Expression Humans Melanocytes / physiology* Mice Mice, Transgenic Monophenol Monooxygenase / genetics* Nucleic Acid Hybridization Promoter Regions, Genetic RNA, Messenger / genetics Retina / physiology* Skin Physiological Phenomena Tumor Cells, Cultured |
| Chemical | |
Reg. No./Substance:
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0/RNA, Messenger; EC 1.14.18.1/Monophenol Monooxygenase |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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