Document Detail


A mouse model of ocular blast injury that induces closed globe anterior and posterior pole damage.
MedLine Citation:
PMID:  22504073     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We developed and characterized a mouse model of primary ocular blast injury. The device consists of: a pressurized air tank attached to a regulated paintball gun with a machined barrel; a chamber that protects the mouse from direct injury and recoil, while exposing the eye; and a secure platform that enables fine, controlled movement of the chamber in relation to the barrel. Expected pressures were calculated and the optimal pressure transducer, based on the predicted pressures, was positioned to measure output pressures at the location where the mouse eye would be placed. Mice were exposed to one of three blast pressures (23.6, 26.4, or 30.4 psi). Gross pathology, intraocular pressure, optical coherence tomography, and visual acuity were assessed 0, 3, 7, 14, and 28 days after exposure. Contralateral eyes and non-blast exposed mice were used as controls. We detected increased damage with increased pressures and a shift in the damage profile over time. Gross pathology included corneal edema, corneal abrasions, and optic nerve avulsion. Retinal damage was detected by optical coherence tomography and a deficit in visual acuity was detected by optokinetics. Our findings are comparable to those identified in Veterans of the recent wars with closed eye injuries as a result of blast exposure. In summary, this is a relatively simple system that creates injuries with features similar to those seen in patients with ocular blast trauma. This is an important new model for testing the short-term and long-term spectrum of closed globe blast injuries and potential therapeutic interventions.
Authors:
Jessica Hines-Beard; Jeffrey Marchetta; Sarah Gordon; Edward Chaum; Eldon E Geisert; Tonia S Rex
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.     Date:  2012-04-07
Journal Detail:
Title:  Experimental eye research     Volume:  99     ISSN:  1096-0007     ISO Abbreviation:  Exp. Eye Res.     Publication Date:  2012 Jun 
Date Detail:
Created Date:  2012-06-04     Completed Date:  2012-08-16     Revised Date:  2014-03-19    
Medline Journal Info:
Nlm Unique ID:  0370707     Medline TA:  Exp Eye Res     Country:  England    
Other Details:
Languages:  eng     Pagination:  63-70     Citation Subset:  IM    
Copyright Information:
Copyright © 2012 Elsevier Ltd. All rights reserved.
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MeSH Terms
Descriptor/Qualifier:
Animals
Anterior Eye Segment / injuries*
Blast Injuries / etiology*,  pathology
Body Weight
Cataract / etiology
Corneal Diseases / etiology
Disease Models, Animal*
Eye Injuries / etiology*,  pathology
Female
Intraocular Pressure / physiology
Mice
Mice, Inbred BALB C
Posterior Eye Segment / injuries*
Tomography, Optical Coherence
Visual Acuity / physiology
Wounds, Nonpenetrating / etiology*,  pathology
Grant Support
ID/Acronym/Agency:
5P30EY13080/EY/NEI NIH HHS; P30 EY013080/EY/NEI NIH HHS; R01 EY017841/EY/NEI NIH HHS; R01 EY022349/EY/NEI NIH HHS; R01EY017841/EY/NEI NIH HHS; R01EY022349/EY/NEI NIH HHS
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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