Document Detail

A mouse leukemia cell mutant resistant to blasticidin S.
MedLine Citation:
PMID:  892936     Owner:  NLM     Status:  MEDLINE    
After nitrosoguanidine mutagenesis we isolated from mouse leukemia L5178Y cells a mutant cell (Bla-R) resistant to blasticidin S, an inhibitor of protein synthesis. Neither growth nor leucine incorporation into hot-acid insoluble fraction of Bla-R cell was inhibited by 5 to 20 microgram/ml blasticidin S, which almost completely blocked protein synthesis as well as growth of the parental L5178Y cells. However, other inhibitors such as fusidic acid, cycloheximide, ricin D or L-asparaginase blocked protein synthesis in Bla-R cells to the same extent as in L5178Y cells. Protein synthesis in vitro using S-30 extracts from the parental cell line L5178Y was almost completely blocked in the presence of the antibiotic, while no inhibition by blasticidin S occurred when S-30 extracts -rom Bla-R mutant cells were used. Protein synthesis assays were made by using the S100 fraction from rat liver together with ribosomes from either L5178Y cells or Bla-R cells. Blasticidin S inhibited protein synthesis when ribosomes were derived from L5178Y cells, but not from Bla-R mutant.
M Kuwano; K Matsui; K Takenaka; S Akiyama; H Endo
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  International journal of cancer. Journal international du cancer     Volume:  20     ISSN:  0020-7136     ISO Abbreviation:  Int. J. Cancer     Publication Date:  1977 Aug 
Date Detail:
Created Date:  1977-10-28     Completed Date:  1977-10-28     Revised Date:  2007-07-24    
Medline Journal Info:
Nlm Unique ID:  0042124     Medline TA:  Int J Cancer     Country:  DENMARK    
Other Details:
Languages:  eng     Pagination:  296-302     Citation Subset:  IM    
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MeSH Terms
Anti-Bacterial Agents / pharmacology*
Asparaginase / pharmacology
Cell Line*
Cycloheximide / pharmacology
Fusidic Acid / pharmacology
Guanidines / pharmacology*
Leukemia, Experimental / immunology*
Mutation / drug effects
Neoplasm Proteins / biosynthesis
Ricin / pharmacology
Reg. No./Substance:
0/Anti-Bacterial Agents; 0/Guanidines; 0/Neoplasm Proteins; 66-81-9/Cycloheximide; 6990-06-3/Fusidic Acid; 9009-86-3/Ricin; EC

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