Document Detail

The motility of normal and cancer cells in response to the combined influence of the substrate rigidity and anisotropic microstructure.
MedLine Citation:
PMID:  18191193     Owner:  NLM     Status:  MEDLINE    
Cell adhesion and migration are strongly influenced by extracellular matrix (ECM) architecture and rigidity, but little is known about the concomitant influence of such environmental signals to cell responses, especially when considering cells of similar origin and morphology, but exhibiting a normal or cancerous phenotype. Using micropatterned polydimethylsiloxane substrates (PDMS) with tunable stiffness (500 kPa, 750 kPa, 2000 kPa) and topography (lines, pillars or unpatterned), we systematically analyse the differential response of normal (3T3) and cancer (SaI/N) fibroblastic cells. Our results demonstrate that both cells exhibit differential morphology and motility responses to changes in substrate rigidity and microtopography. 3T3 polarisation and spreading are influenced by substrate microtopography and rigidity. The cells exhibit a persistent type of migration, which depends on the substrate anisotropy. In contrast, the dynamic of SaI/N spreading is strongly modified by the substrate topography but not by substrate rigidity. SaI/N morphology and migration seem to escape from extracellular cues: the cells exhibit uncorrelated migration trajectories and a large dispersion of their migration speed, which increases with substrate rigidity.
Tzvetelina Tzvetkova-Chevolleau; Angélique Stéphanou; David Fuard; Jacques Ohayon; Patrick Schiavone; Philippe Tracqui
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Publication Detail:
Type:  Journal Article     Date:  2008-01-11
Journal Detail:
Title:  Biomaterials     Volume:  29     ISSN:  0142-9612     ISO Abbreviation:  Biomaterials     Publication Date:  2008 Apr 
Date Detail:
Created Date:  2008-01-29     Completed Date:  2008-07-11     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8100316     Medline TA:  Biomaterials     Country:  England    
Other Details:
Languages:  eng     Pagination:  1541-51     Citation Subset:  IM    
LTM/CNRS/UMR5129, c/o CEA Grenoble, 17 rue des Martyrs, 38054 Grenoble cedex 9, France. <>
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MeSH Terms
Cell Adhesion / drug effects
Cell Line, Tumor
Cell Movement*
Dimethylpolysiloxanes / chemistry*,  pharmacology
Extracellular Matrix / chemistry*,  ultrastructure
Microscopy, Electron, Scanning
NIH 3T3 Cells
Silicones / chemistry*,  pharmacology
Reg. No./Substance:
0/Dimethylpolysiloxanes; 0/Silicones; 63148-62-9/baysilon

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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