Document Detail

The morphological changes in cultured cells caused by Bordetella pertussis adenylate cyclase toxin.
MedLine Citation:
PMID:  18179583     Owner:  NLM     Status:  MEDLINE    
Bordetella pertussis is the causative agent for human whooping cough. It was found that Bordetella pertussis infection caused a change in shape from flat to round in L2 cells, which are derived from rat type 2 alveolar cells. This phenomenon was reproduced using the culture supernatant of B. pertussis, and bacterium-free adenylate cyclase toxin (CyaA) was identified as the factor responsible. A purified preparation of wild-type CyaA but not an enzyme-dead mutant caused the cell rounding. It was examined whether CyaA causes similar morphological changes in various cultured cell lines. L2, EBL, HEK293T, MC3T3-E1, NIH 3T3, and Vero cells were rounded by the toxin whereas Caco-2, Eph4, and MDCK cells were not, although all these cells showed a significant elevation of the intracellular cAMP level in response to CyaA treatment, which indicates that there is no quantitative correlation between the rounding phenotype and the intracellular cAMP level. CyaA has been believed to target various immunocompetent cells and support the establishment of the bacterial infection by subverting the host immune responses. The possibility that CyaA may also affect tissue cells such as respiratory epithelial cells and may be involved in the pathogenesis of the bacterial infection is also indicated.
Hiroe Ohnishi; Masami Miyake; Shigeki Kamitani; Yasuhiko Horiguchi
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2007-12-19
Journal Detail:
Title:  FEMS microbiology letters     Volume:  279     ISSN:  0378-1097     ISO Abbreviation:  FEMS Microbiol. Lett.     Publication Date:  2008 Feb 
Date Detail:
Created Date:  2008-01-25     Completed Date:  2008-04-03     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7705721     Medline TA:  FEMS Microbiol Lett     Country:  England    
Other Details:
Languages:  eng     Pagination:  174-9     Citation Subset:  IM    
Department of Molecular Bacteriology, Research Institute for Microbial Diseases, Osaka University, 3-1 Yamadaoka, Suita, Osaka, Japan.
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MeSH Terms
Adenylate Cyclase Toxin / toxicity*
Bordetella pertussis / physiology*
Cell Line
Cell Shape / drug effects*
Cells, Cultured
Cercopithecus aethiops
Cyclic AMP / analysis
Reg. No./Substance:
0/Adenylate Cyclase Toxin; 60-92-4/Cyclic AMP

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