Document Detail


The morphological and biochemical response of avian embryonic sympathoadrenal cells to nerve growth factor is developmentally regulated.
MedLine Citation:
PMID:  12888213     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Cellular differentiation is a stepwise process where environmental factors are essential key components to direct cells to their final phenotype. The sympathoadrenal (SA) system is one of the principal models used to study the role of environmental factors in the development of the peripheral nervous system. Two major cell types originate from the SA progenitor: the principal neurons of the sympathetic ganglia and the chromaffin cells of the adrenal medulla. These cells are directed to their final phenotype by a series of environmental factors, of which nerve growth factor (NGF) and glucocorticoids, are the best studied. Previously, we have shown that 11-day embryonic chick sympathetic cell cultures increased their neuropeptide Y (NPY) protein and mRNA levels in the presence of NGF. In contrast, NGF had no such effect in chromaffin cell cultures from the same developmental stage. These results were unexpected since both cells types respond morphologically to NGF. To determine if these cells can gain or lose their capacity to respond to NGF, morphological and biochemical studies were done at earlier stages using immunocytochemical, radioimmunoassay and polymerase chain reaction (PCR) techniques. Interestingly we found that in E-7 chromaffin cells there is a biochemical and morphological response to NGF, while E-7 sympathetic cells lack this response. Our observations show a developmental point of regulation of morphological and biochemical properties by NGF and reveal an age dependent capacity of SA cells to acquire or lose competence to an environmental factor.
Authors:
Jennifer L Barreto-Estrada; Wanda E Medina-Ortíz; José E García-Arrarás
Related Documents :
8359593 - Ngf retards apoptosis in chick embryo bursal cell in vitro.
12102543 - The role of dopamine in manganese-induced oxidative injury in rat pheochromocytoma cells.
15948253 - Cell cycle arrest and apoptotic cell death in cultured human gastric carcinoma cells me...
Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Brain research. Developmental brain research     Volume:  144     ISSN:  0165-3806     ISO Abbreviation:  Brain Res. Dev. Brain Res.     Publication Date:  2003 Aug 
Date Detail:
Created Date:  2003-07-30     Completed Date:  2003-10-03     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  8908639     Medline TA:  Brain Res Dev Brain Res     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  1-8     Citation Subset:  IM    
Affiliation:
Department of Biology, University of Puerto Rico, P.O. Box 23360, UPR Station, Rio Piedras, Puerto Rico, PR, 00931-3360, USA.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Adrenal Medulla / cytology,  embryology,  metabolism*
Animals
Cell Count / methods
Cell Size / physiology
Cells, Cultured
Chick Embryo
Chromaffin Cells / chemistry,  metabolism*
Cyclophilins / genetics,  metabolism
Ganglia, Sympathetic / cytology,  embryology,  metabolism*
Nerve Growth Factor / physiology*
Neurites / physiology
Neurons / chemistry,  metabolism*
Neuropeptide Y / chemistry,  genetics,  metabolism
Ovum
RNA, Messenger / biosynthesis
Radioimmunoassay
Reverse Transcriptase Polymerase Chain Reaction / methods
Stem Cells / metabolism
Time Factors
Grant Support
ID/Acronym/Agency:
RR0-3641-01/RR/NCRR NIH HHS; S06 GM08102/GM/NIGMS NIH HHS
Chemical
Reg. No./Substance:
0/Neuropeptide Y; 0/RNA, Messenger; 9061-61-4/Nerve Growth Factor; EC 5.2.1.-/Cyclophilins

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Application of high-performance liquid chromatography-mass spectrometry to detection of diuretics in...
Next Document:  Identity and neuroanatomical localization of messenger RNAs that change expression in the neural tub...