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The monocyte transcriptome during pregnancy in multiple sclerosis: prominent expression of the Fc-receptor CD64.
MedLine Citation:
PMID:  21135020     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
Background: During the third trimester of pregnancy multiple sclerosis (MS) disease activity is reduced. It is not fully understood which factors mediate this disease amelioration. Objective: To study alterations of the monocyte transcriptome during pregnancy in MS patients, using a genomewide approach to identify differentially regulated genes. Methods: Women with MS and healthy controls were longitudinally studied, including a visit before pregnancy. Results: RNA-microarray analysis was performed in six patients. We found a significant increase of CD64 (Fc gamma receptor 1a, FcgR1a) during the third trimester compared with baseline, confirmed by RT-PCR in a group of ten patients. Analysis with Ingenuity software was performed using all genes expression of which was altered at least 1.5-fold in at least five out of six patients. Major networks that were altered during MS pregnancy were: cell-to-cell signalling and interaction, immune response, and cell signalling. From the genes selected for Ingenuity analysis, seven additional candidate genes, selected for their biological interest, were tested using RT-PCR in ten patients with MS and nine controls. We found an increased expression of JAK2 and STAT1 directly postpartum in patients with MS and in controls. Conclusion: The increased CD64 expression during pregnancy is indicative of enhanced innate immune functions.
Authors:
Rinze F Neuteboom; Evert Verbraak; Annet F Wierenga-Wolf; Jane Sa Voerman; Marjan van Meurs; Sigrid M Swagemakers; Peter J van de Spek; Eric Ap Steegers; Christianne Jm de Groot; Jon D Laman; Rogier Q Hintzen
Publication Detail:
Type:  Journal Article     Date:  2010-12-06
Journal Detail:
Title:  Multiple sclerosis (Houndmills, Basingstoke, England)     Volume:  17     ISSN:  1477-0970     ISO Abbreviation:  Mult. Scler.     Publication Date:  2011 Apr 
Date Detail:
Created Date:  2011-04-13     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9509185     Medline TA:  Mult Scler     Country:  England    
Other Details:
Languages:  eng     Pagination:  389-96     Citation Subset:  IM    
Affiliation:
1Department of Neurology, Erasmus MC, Erasmus University Medical Centre, Rotterdam, The Netherlands.
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