| The monocyte transcriptome during pregnancy in multiple sclerosis: prominent expression of the Fc-receptor CD64. | |
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MedLine Citation:
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PMID: 21135020 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
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Background: During the third trimester of pregnancy multiple sclerosis (MS) disease activity is reduced. It is not fully understood which factors mediate this disease amelioration. Objective: To study alterations of the monocyte transcriptome during pregnancy in MS patients, using a genomewide approach to identify differentially regulated genes. Methods: Women with MS and healthy controls were longitudinally studied, including a visit before pregnancy. Results: RNA-microarray analysis was performed in six patients. We found a significant increase of CD64 (Fc gamma receptor 1a, FcgR1a) during the third trimester compared with baseline, confirmed by RT-PCR in a group of ten patients. Analysis with Ingenuity software was performed using all genes expression of which was altered at least 1.5-fold in at least five out of six patients. Major networks that were altered during MS pregnancy were: cell-to-cell signalling and interaction, immune response, and cell signalling. From the genes selected for Ingenuity analysis, seven additional candidate genes, selected for their biological interest, were tested using RT-PCR in ten patients with MS and nine controls. We found an increased expression of JAK2 and STAT1 directly postpartum in patients with MS and in controls. Conclusion: The increased CD64 expression during pregnancy is indicative of enhanced innate immune functions. |
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Authors:
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Rinze F Neuteboom; Evert Verbraak; Annet F Wierenga-Wolf; Jane Sa Voerman; Marjan van Meurs; Sigrid M Swagemakers; Peter J van de Spek; Eric Ap Steegers; Christianne Jm de Groot; Jon D Laman; Rogier Q Hintzen |
Publication Detail:
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Type: Journal Article Date: 2010-12-06 |
Journal Detail:
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Title: Multiple sclerosis (Houndmills, Basingstoke, England) Volume: 17 ISSN: 1477-0970 ISO Abbreviation: Mult. Scler. Publication Date: 2011 Apr |
Date Detail:
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Created Date: 2011-04-13 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9509185 Medline TA: Mult Scler Country: England |
Other Details:
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Languages: eng Pagination: 389-96 Citation Subset: IM |
Affiliation:
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1Department of Neurology, Erasmus MC, Erasmus University Medical Centre, Rotterdam, The Netherlands. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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