Document Detail


A monoclonal antibody to vasopressin: preparation, characterization, and application in immunocytochemistry.
MedLine Citation:
PMID:  6185560     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The hypothalamo-neurohypophysial system, containing the hormones oxytocin (OT) and vasopressin (VP) and their associated carrier proteins, the neurophysins (NPS), has been the subject of extensive investigation for more than 40 years. This system has been reinvestigated during the last decade by application of immunocytochemical methods employing the rabbit antisera to the hormones and NPS. In this study we describe the preparation and characterization of a monoclonal antibody to VP and its application in immunohistochemistry. The antibody did not cross-react with OT or arginine vasotocin (AVT). Its antigenic determinants as characterized by absorption with various VP analogs included two aromatic amino acids: Phe in position 3, and to a lesser extent Tyr in 2. Tissue fixation with formaldehyde resulted in inadequate immunostaining as compared to glutaraldehyde, most likely due to interference with the aromatic amino acid determinants by the former fixative.
Authors:
A Hou-Yu; P H Ehrlich; G Valiquette; D L Engelhardt; W H Sawyer; G Nilaver; E A Zimmerman
Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society     Volume:  30     ISSN:  0022-1554     ISO Abbreviation:  J. Histochem. Cytochem.     Publication Date:  1982 Dec 
Date Detail:
Created Date:  1983-03-24     Completed Date:  1983-03-24     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  9815334     Medline TA:  J Histochem Cytochem     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1249-60     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Antibodies, Monoclonal*
Antibody Specificity
Arginine Vasopressin / analysis,  immunology*
Brain Chemistry*
Epitopes
Fixatives
Glutaral
Hybridomas
Hypothalamus / analysis
Immunoenzyme Techniques
Mice
Paraventricular Hypothalamic Nucleus / analysis
Rats
Suprachiasmatic Nucleus / analysis
Grant Support
ID/Acronym/Agency:
AM 01940/AM/NIADDK NIH HHS; AM 20337/AM/NIADDK NIH HHS; HL 24105/HL/NHLBI NIH HHS
Chemical
Reg. No./Substance:
0/Antibodies, Monoclonal; 0/Epitopes; 0/Fixatives; 111-30-8/Glutaral; 113-79-1/Arginine Vasopressin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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