Document Detail


A monoclonal antibody to the human leukocyte adhesion molecule intercellular adhesion molecule-2. Cellular distribution and molecular characterization of the antigen.
MedLine Citation:
PMID:  2016518     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The DNA of the human leukocyte adhesion molecule intercellular adhesion molecule-2 (ICAM-2) was synthesized by the polymerase chain reaction, and a protein A-ICAM-2 fusion protein was expressed in Escherichia coli. The fusion protein was used as immunogen to obtain mAb to ICAM-2. The 6D5 antibody was selected by its reactivity in immunofluorescence with the endothelial cell line Eahy926. The antibody precipitated a 55,000 m.w. glycoprotein from radioactivity surface labeled cells and also reacted in Western blotting. As measured by immunofluorescence flow cytometry, the antibody reacted with lymphoblastoid B cells of normal origin, some Burkitt lymphoma cell lines, vascular endothelial cells, the endothelial cell line Eahy926, 4 and a subpopulation of Con A-stimulated blood mononuclear cells. The two Ig-like domains of ICAM-2 were separately expressed in E. coli, and the antibody was shown to react with the NH2-terminal domain. The antibody inhibited the CD11/CD18-dependent binding of HL-60 promyelocytic leukemia cells to transfected COS-1 cells.
Authors:
P Nortamo; R Salcedo; T Timonen; M Patarroyo; C G Gahmberg
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of immunology (Baltimore, Md. : 1950)     Volume:  146     ISSN:  0022-1767     ISO Abbreviation:  J. Immunol.     Publication Date:  1991 Apr 
Date Detail:
Created Date:  1991-05-21     Completed Date:  1991-05-21     Revised Date:  2013-02-25    
Medline Journal Info:
Nlm Unique ID:  2985117R     Medline TA:  J Immunol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  2530-5     Citation Subset:  AIM; IM    
Affiliation:
Department of Biochemistry, University of Helsinki, Finland.
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MeSH Terms
Descriptor/Qualifier:
Antibodies, Monoclonal / immunology*
Antigens, CD*
B-Lymphocytes / immunology
Base Sequence
Blotting, Western
Cell Adhesion / immunology
Cell Adhesion Molecules / analysis,  immunology*
Cell Line
Flow Cytometry
Fluorescent Antibody Technique
Humans
Leukocytes / immunology
Molecular Sequence Data
Transfection
Chemical
Reg. No./Substance:
0/Antibodies, Monoclonal; 0/Antigens, CD; 0/Cell Adhesion Molecules; 0/ICAM2 protein, human

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