Document Detail

A monoclonal antibody to the human leukocyte adhesion molecule intercellular adhesion molecule-2. Cellular distribution and molecular characterization of the antigen.
MedLine Citation:
PMID:  2016518     Owner:  NLM     Status:  MEDLINE    
The DNA of the human leukocyte adhesion molecule intercellular adhesion molecule-2 (ICAM-2) was synthesized by the polymerase chain reaction, and a protein A-ICAM-2 fusion protein was expressed in Escherichia coli. The fusion protein was used as immunogen to obtain mAb to ICAM-2. The 6D5 antibody was selected by its reactivity in immunofluorescence with the endothelial cell line Eahy926. The antibody precipitated a 55,000 m.w. glycoprotein from radioactivity surface labeled cells and also reacted in Western blotting. As measured by immunofluorescence flow cytometry, the antibody reacted with lymphoblastoid B cells of normal origin, some Burkitt lymphoma cell lines, vascular endothelial cells, the endothelial cell line Eahy926, 4 and a subpopulation of Con A-stimulated blood mononuclear cells. The two Ig-like domains of ICAM-2 were separately expressed in E. coli, and the antibody was shown to react with the NH2-terminal domain. The antibody inhibited the CD11/CD18-dependent binding of HL-60 promyelocytic leukemia cells to transfected COS-1 cells.
P Nortamo; R Salcedo; T Timonen; M Patarroyo; C G Gahmberg
Related Documents :
1381388 - Mechanism of peripheral t cell activation by coengagement of cd44 and cd2.
1706378 - Cd40 signaling activates cd11a/cd18 (lfa-1)-mediated adhesion in b cells.
9530558 - Determination of t-lymphocyte adhesion to endothelial cells using a novel luminescence ...
16269618 - Dynamic shifts in lfa-1 affinity regulate neutrophil rolling, arrest, and transmigratio...
2484708 - Sin-1 stimulates the production of cyclic gmp but not cyclic amp in porcine aortic endo...
12609838 - Platelet factor 4 enhances generation of activated protein c in vitro and in vivo.
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of immunology (Baltimore, Md. : 1950)     Volume:  146     ISSN:  0022-1767     ISO Abbreviation:  J. Immunol.     Publication Date:  1991 Apr 
Date Detail:
Created Date:  1991-05-21     Completed Date:  1991-05-21     Revised Date:  2013-02-25    
Medline Journal Info:
Nlm Unique ID:  2985117R     Medline TA:  J Immunol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  2530-5     Citation Subset:  AIM; IM    
Department of Biochemistry, University of Helsinki, Finland.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Antibodies, Monoclonal / immunology*
Antigens, CD*
B-Lymphocytes / immunology
Base Sequence
Blotting, Western
Cell Adhesion / immunology
Cell Adhesion Molecules / analysis,  immunology*
Cell Line
Flow Cytometry
Fluorescent Antibody Technique
Leukocytes / immunology
Molecular Sequence Data
Reg. No./Substance:
0/Antibodies, Monoclonal; 0/Antigens, CD; 0/Cell Adhesion Molecules; 0/ICAM2 protein, human

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  An improved microphotometry system for measurement of cytochrome P-450 in hepatocyte cytoplasm.
Next Document:  The mitogenic activity of type III bacterial Ig binding proteins (protein G) for human peripheral bl...