Document Detail


A monoclonal antibody reactive with terminal lactotriaosyl residue-containing oligosaccharides and its application to characterizing cell surface expression of the glyco-epitopes in COS-1 cells.
MedLine Citation:
PMID:  8280116     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A new monoclonal antibody (MoAb), designated JF12, reactive strongly with Lc3 (GlcNAc beta 1-->3Gal beta 1-->4Glc beta 1-->Cer) but only slightly with nLc5 (GlcNAc beta 1-->3Gal beta 1-->4GlcNAc beta 1-->3Gal beta 1-->4Glc beta 1-->Cer) has been prepared after immunization of Balb/c mice with Lc3. By the flowcytometrical analyses of COS-1 cells, which was strongly stained with JF12, the reactivity completely disappeared in the confluent condition upon harvesting by the protease treatments of the cell surface. In the sparse condition, however, the cells still retained JF12 reactivity in spite of the protease treatments. This strongly suggests that the expression of terminal GlcNAc-containing glycoconjugates on the surface of COS-1 cells may be dramatically modulated by protease-sensitive membranous components being dependent upon the cell density.
Authors:
M Nakamura; A Tsunoda; K Sakoe; M Saito
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Biochemical and biophysical research communications     Volume:  197     ISSN:  0006-291X     ISO Abbreviation:  Biochem. Biophys. Res. Commun.     Publication Date:  1993 Dec 
Date Detail:
Created Date:  1994-02-10     Completed Date:  1994-02-10     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0372516     Medline TA:  Biochem Biophys Res Commun     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1025-33     Citation Subset:  IM    
Affiliation:
Division of Hemopoiesis, Jichi Medical School, Tochigi, Japan.
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MeSH Terms
Descriptor/Qualifier:
Animals
Antibodies, Monoclonal
Carbohydrate Conformation
Carbohydrate Sequence
Cell Line
Cell Membrane / metabolism
Cercopithecus aethiops
Endopeptidases
Enzyme-Linked Immunosorbent Assay
Flow Cytometry
Fluorescent Antibody Technique
Glycopeptides / analysis,  biosynthesis*
Lactosylceramides / analysis*,  chemistry
Mice
Mice, Inbred BALB C / immunology
Molecular Sequence Data
Oligosaccharides / analysis*,  chemistry
Transfection
Chemical
Reg. No./Substance:
0/Antibodies, Monoclonal; 0/Glycopeptides; 0/Lactosylceramides; 0/Oligosaccharides; 73467-80-8/lactotriaosylceramide; 97666-64-3/N-acetylglucosaminyl-beta-1-3-galactosyl-1-4-(N-acetylglucosaminyl)-1-3-galactosyl-1-4-glucosyl-1-ceramide; EC 3.4.-/Endopeptidases

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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