Document Detail

A monochromatic action spectrum for the photoinduction of the UV-absorbing mycosporine-like amino acid shinorine in the red alga Chondrus crispus.
MedLine Citation:
PMID:  15291302     Owner:  NLM     Status:  MEDLINE    
To determine the action spectrum for photoinduction of the ultraviolet (UV)-absorbing mycosporine-like amino acid shinorine, specimens of the marine red alga Chondrus crispus were irradiated with monochromatic light of various wavelengths using the Okazaki large spectrograph at the National Institute for Basic Biology, Okazaki, Japan. Fluence response curves were determined for the wavelengths between 280 and 750 nm, by irradiating the algae with monochromatic light for 10 h, followed by 4 h of 25 micromol m(-2) s(-1) photosynthetically active radiation and 10 h darkness. Samples were taken after the second exposure interval. A linear correlation between fluence rate and accumulated shinorine concentration was detected for wavelengths between 350 and 490 nm in the fluence rate range of 20-30 micromol m(-2) s(-1), whereas there was no induction above 490 nm. Below 350 nm a decline in shinorine concentration could be observed at fluence rates above 30 micromol m(-2) s(-1), probably due to an inhibition of photosynthetic activity and a subsequent impairment of shinorine biosynthesis. The constructed action spectrum indicated that the photoreceptors mediating shinorine photoinduction might be an unidentified UV-A-type photoreceptor with absorption peaks at 320, 340 and 400 nm.
Gudrun Kräbs; Masakatsu Watanabe; Christian Wiencke
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Photochemistry and photobiology     Volume:  79     ISSN:  0031-8655     ISO Abbreviation:  Photochem. Photobiol.     Publication Date:  2004 Jun 
Date Detail:
Created Date:  2004-08-04     Completed Date:  2004-09-07     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0376425     Medline TA:  Photochem Photobiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  515-9     Citation Subset:  IM    
Alfred Wegener Institute for Polar and Marine Research, Bremerhaven, Germany.
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MeSH Terms
Algae, Red / metabolism*,  radiation effects*
Amino Acids / biosynthesis,  metabolism*
Dose-Response Relationship, Radiation
Species Specificity
Ultraviolet Rays*
Reg. No./Substance:
0/Amino Acids

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