Document Detail

The molecular weight forms of rat growth hormone secreted in response to growth hormone-releasing factor.
MedLine Citation:
PMID:  3083419     Owner:  NLM     Status:  MEDLINE    
The different molecular weight forms of immunoreactive growth hormone (irGH) secreted by the anterior pituitary of rats were evaluated during basal and stimulated secretion in vitro and in vivo. Anterior pituitary cells maintained in a monolayer culture system secreted only a 22,000-Da form of GH based on Sephacryl S-200 column chromatography. This was also true for the irGH secreted in response to growth hormone-releasing factor and prostaglandin E2 stimulation. In contrast, the molecular weight forms of irGH found in plasma under basal conditions included an approximately 90,000-Da form as well as the expected 22,000-Da form. The concentrations of both forms increased following growth hormone-releasing factor stimulation although there was a shift in the ratio of the forms secreted. These results suggest that the larger molecular weight forms of rat GH observed in plasma may be the result of some postsecretory process which occurs in blood and suggests a possible regulatory function for the larger molecular weight forms as it pertains to the bioavailability of GH in vivo.
W B Wehrenberg; P Chatelain; A Baird
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Proceedings of the Society for Experimental Biology and Medicine. Society for Experimental Biology and Medicine (New York, N.Y.)     Volume:  182     ISSN:  0037-9727     ISO Abbreviation:  Proc. Soc. Exp. Biol. Med.     Publication Date:  1986 May 
Date Detail:
Created Date:  1986-05-16     Completed Date:  1986-05-16     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  7505892     Medline TA:  Proc Soc Exp Biol Med     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  107-11     Citation Subset:  IM    
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MeSH Terms
Cells, Cultured
Chromatography, Gel
Growth Hormone / isolation & purification,  secretion*
Growth Hormone-Releasing Hormone / pharmacology*
Molecular Weight
Pituitary Gland, Anterior / drug effects,  secretion
Rats, Inbred Strains
Grant Support
Reg. No./Substance:
9002-72-6/Growth Hormone; 9034-39-3/Growth Hormone-Releasing Hormone

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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