Document Detail


The molecular weight forms of rat growth hormone secreted in response to growth hormone-releasing factor.
MedLine Citation:
PMID:  3083419     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The different molecular weight forms of immunoreactive growth hormone (irGH) secreted by the anterior pituitary of rats were evaluated during basal and stimulated secretion in vitro and in vivo. Anterior pituitary cells maintained in a monolayer culture system secreted only a 22,000-Da form of GH based on Sephacryl S-200 column chromatography. This was also true for the irGH secreted in response to growth hormone-releasing factor and prostaglandin E2 stimulation. In contrast, the molecular weight forms of irGH found in plasma under basal conditions included an approximately 90,000-Da form as well as the expected 22,000-Da form. The concentrations of both forms increased following growth hormone-releasing factor stimulation although there was a shift in the ratio of the forms secreted. These results suggest that the larger molecular weight forms of rat GH observed in plasma may be the result of some postsecretory process which occurs in blood and suggests a possible regulatory function for the larger molecular weight forms as it pertains to the bioavailability of GH in vivo.
Authors:
W B Wehrenberg; P Chatelain; A Baird
Related Documents :
2890079 - Hypophysectomy does not alter the effects of somatostatin or growth hormone on canine s...
11438399 - Selection for growth rate or against back fat thickness in pigs is associated with chan...
16442609 - Ovariectomy during the luteal phase influences secretion of prolactin, growth hormone, ...
1671389 - Growth hormone (gh) autofeedback on gh response to gh-releasing hormone. role of free f...
16298769 - Diurnal changes in blood metabolites and their relation to plasma growth hormone and ti...
12537759 - Pituitary adenoma in carney complex: an immunohistochemical, ultrastructural, and immun...
12530049 - The significance of tenascin-c serum level as tumor marker in squamous cell carcinoma o...
8862789 - Effects of gonadotropin treatment and withdrawal on follicular growth, cell proliferati...
12398559 - Comparison of plasma concentrations for two amiodarone products.
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Proceedings of the Society for Experimental Biology and Medicine. Society for Experimental Biology and Medicine (New York, N.Y.)     Volume:  182     ISSN:  0037-9727     ISO Abbreviation:  Proc. Soc. Exp. Biol. Med.     Publication Date:  1986 May 
Date Detail:
Created Date:  1986-05-16     Completed Date:  1986-05-16     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  7505892     Medline TA:  Proc Soc Exp Biol Med     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  107-11     Citation Subset:  IM    
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Animals
Cells, Cultured
Chromatography, Gel
Growth Hormone / isolation & purification,  secretion*
Growth Hormone-Releasing Hormone / pharmacology*
Humans
Male
Molecular Weight
Pituitary Gland, Anterior / drug effects,  secretion
Radioimmunoassay
Rats
Rats, Inbred Strains
Grant Support
ID/Acronym/Agency:
AM-18811/AM/NIADDK NIH HHS; HD-09690/HD/NICHD NIH HHS
Chemical
Reg. No./Substance:
9002-72-6/Growth Hormone; 9034-39-3/Growth Hormone-Releasing Hormone

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Use of specific antibodies to quantitate the guanine nucleotide-binding protein Go in brain.
Next Document:  Changes in pituitary prolactin responsiveness to TRH during pregnancy.