| The molecular selectivity of phospholipase D in HL60 granulocytes. | |
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MedLine Citation:
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PMID: 7758577 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The molecular selectivity of PLD in PMA-stimulated HL60 granulocytes was determined by HPLC analysis of [3H]butanol incorporation into phosphatidyl[3H]butanol (Ptd[3H]But) molecular species. Comparison with phospholipid compositions confirmed that PLD acted primarily on phosphatidylcholine (PtdCho). Apparent enzyme selectivity was suggested by negligible formation of PB16:0/16:0 and preferential synthesis of Ptd[3H]But species containing sn-1 18:0. Culture with exogenous 18:2n-6 or 20:4n-6 readily modified both PtdCho and Ptd[3H]But compositions, and accentuated the apparent selectivity of stimulated PLD for sn-1 18:0 species of PtdCho. Such modifications to PLD-based signalling mechanisms may contribute to the modulatory effects of altered dietary lipid intakes on cellular functions. |
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Authors:
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Y M Heung; A D Postle |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: FEBS letters Volume: 364 ISSN: 0014-5793 ISO Abbreviation: FEBS Lett. Publication Date: 1995 May |
Date Detail:
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Created Date: 1995-06-26 Completed Date: 1995-06-26 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0155157 Medline TA: FEBS Lett Country: NETHERLANDS |
Other Details:
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Languages: eng Pagination: 250-4 Citation Subset: IM |
Affiliation:
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University of Southampton, Southampton General Hospital, UK. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Butanols
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metabolism Cell Line Chromatography, High Pressure Liquid Glycerophospholipids* Granulocytes / enzymology* Humans Phosphatidic Acids / metabolism Phosphatidylcholines / metabolism Phospholipase D / metabolism* Substrate Specificity Tetradecanoylphorbol Acetate / pharmacology Tritium |
| Chemical | |
Reg. No./Substance:
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0/Butanols; 0/Glycerophospholipids; 0/Phosphatidic Acids; 0/Phosphatidylcholines; 0/phosphatidylbutanol; 10028-17-8/Tritium; 16561-29-8/Tetradecanoylphorbol Acetate; EC 3.1.4.4/Phospholipase D |
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