Document Detail

The molecular function of the yeast polo-like kinase Cdc5 in Cdc14 release during early anaphase.
MedLine Citation:
PMID:  19570916     Owner:  NLM     Status:  MEDLINE    
In the budding yeast Saccharomyces cerevisiae, Cdc14 is sequestered within the nucleolus before anaphase entry through its association with Net1/Cfi1, a nucleolar protein. Protein phosphatase PP2A(Cdc55) dephosphorylates Net1 and keeps it as a hypophosphorylated form before anaphase. Activation of the Cdc fourteen early anaphase release (FEAR) pathway after anaphase entry induces a brief Cdc14 release from the nucleolus. Some of the components in the FEAR pathway, including Esp1, Slk19, and Spo12, inactivate PP2A(Cdc55), allowing the phosphorylation of Net1 by mitotic cyclin-dependent kinase (Cdk) (Clb2-Cdk1). However, the function of another FEAR component, the Polo-like kinase Cdc5, remains elusive. Here, we show evidence indicating that Cdc5 promotes Cdc14 release primarily by stimulating the degradation of Swe1, the inhibitory kinase for mitotic Cdk. First, we found that deletion of SWE1 partially suppresses the FEAR defects in cdc5 mutants. In contrast, high levels of Swe1 impair FEAR activation. We also demonstrated that the accumulation of Swe1 in cdc5 mutants is responsible for the decreased Net1 phosphorylation. Therefore, we conclude that the down-regulation of Swe1 protein levels by Cdc5 promotes FEAR activation by relieving the inhibition on Clb2-Cdk1, the kinase for Net1 protein.
Fengshan Liang; Fengzhi Jin; Hong Liu; Yanchang Wang
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2009-07-01
Journal Detail:
Title:  Molecular biology of the cell     Volume:  20     ISSN:  1939-4586     ISO Abbreviation:  Mol. Biol. Cell     Publication Date:  2009 Aug 
Date Detail:
Created Date:  2009-08-17     Completed Date:  2009-12-15     Revised Date:  2012-06-12    
Medline Journal Info:
Nlm Unique ID:  9201390     Medline TA:  Mol Biol Cell     Country:  United States    
Other Details:
Languages:  eng     Pagination:  3671-9     Citation Subset:  IM    
Department of Biomedical Sciences, Florida State University, Tallahassee, 32306, USA.
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MeSH Terms
Anaphase / physiology*
CDC2 Protein Kinase / genetics,  metabolism
Cell Cycle Proteins / genetics,  metabolism*
Cell Nucleolus / metabolism
Cyclin B / genetics,  metabolism
DNA, Ribosomal / metabolism
Nuclear Proteins / genetics,  metabolism
Protein Kinases / genetics,  metabolism*
Protein Tyrosine Phosphatases / genetics,  metabolism*
Protein-Tyrosine Kinases / genetics,  metabolism
Recombinant Fusion Proteins / genetics,  metabolism
Saccharomyces cerevisiae / cytology*,  genetics,  metabolism*
Saccharomyces cerevisiae Proteins / genetics,  metabolism*
Signal Transduction / physiology
Reg. No./Substance:
0/CDC14 protein, S cerevisiae; 0/CLB1 protein, S cerevisiae; 0/Cell Cycle Proteins; 0/Cyclin B; 0/DNA, Ribosomal; 0/Net1 protein, S cerevisiae; 0/Nuclear Proteins; 0/Recombinant Fusion Proteins; 0/Saccharomyces cerevisiae Proteins; EC 2.7.-/Protein Kinases; EC 2.7.1.-/SWE1 protein, S cerevisiae; EC Kinases; EC protein, S cerevisiae; EC Protein Kinase; EC Tyrosine Phosphatases

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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