Document Detail


A molecular epidemiological study of Mycobacterium simiae isolated from AIDS patients in Guadeloupe.
MedLine Citation:
PMID:  10921982     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A molecular epidemiological study of Mycobacterium simiae strains isolated from AIDS patients in Guadeloupe was performed by the random amplification of polymorphic DNA (RAPD) and pulsed-field gel electrophoresis (PFGE) of DraI- or XbaI-digested bacterial DNAs. A comparison of RAPD profiles suggested a similarity of banding patterns within a group of patients (two clusters of two and three patients), but the available epidemiological and clinical information did not support this finding. PFGE, on the other hand, showed that all the patients were contaminated with individual isolates. Combined numerical analysis performed by compiling the PFGE patterns obtained after XbaI and DraI digestions of bacterial DNAs suggested the occurrence of polyclonal infection in three of nine patients. Our results do not support a common source of M. simiae infection in Guadeloupe.
Authors:
E Legrand; A Devallois; L Horgen; N Rastogi
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of clinical microbiology     Volume:  38     ISSN:  0095-1137     ISO Abbreviation:  J. Clin. Microbiol.     Publication Date:  2000 Aug 
Date Detail:
Created Date:  2000-09-20     Completed Date:  2000-09-20     Revised Date:  2013-04-17    
Medline Journal Info:
Nlm Unique ID:  7505564     Medline TA:  J Clin Microbiol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  3080-4     Citation Subset:  IM; X    
Affiliation:
Unité de la Tuberculose et des Mycobactéries, Institut Pasteur, F-97165 Pointe à Pitre-Cedex, Guadeloupe.
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MeSH Terms
Descriptor/Qualifier:
AIDS-Related Opportunistic Infections / epidemiology*,  microbiology
Adult
Aged
Electrophoresis, Gel, Pulsed-Field
Female
Guadeloupe / epidemiology
Humans
Male
Middle Aged
Molecular Epidemiology
Mycobacterium / genetics*,  isolation & purification
Mycobacterium Infections / epidemiology*,  microbiology
Random Amplified Polymorphic DNA Technique
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