Document Detail


The molecular basis of host adaptation in cactophilic Drosophila: molecular evolution of a glutathione S-transferase gene (GstD1) in Drosophila mojavensis.
MedLine Citation:
PMID:  18245335     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Drosophila mojavensis is a cactophilic fly endemic to the northwestern deserts of North America. This species includes four genetically isolated cactus host races each individually specializing on the necrotic tissues of a different cactus species. The necrosis of each cactus species provides the resident D. mojavensis populations with a distinct chemical environment. A previous investigation of the role of transcriptional variation in the adaptation of D. mojavensis to its hosts produced a set of candidate loci that are differentially expressed in response to host shifts, and among them was glutathione S-transferase D1 (GstD1). In both D. melanogaster and Anopheles gambiae, GstD1 has been implicated in the resistance of these species to the insecticide dichloro-diphenyl-trichloroethane (DDT). The pattern of sequence variation of the GstD1 locus from all four D. mojavensis populations, D. arizonae (sister species), and D. navojoa (outgroup) has been examined. The data suggest that in two populations of D. mojavensis GstD1 has gone through a period of adaptive amino acid evolution. Further analyses indicate that of the seven amino acid fixations that occurred in the D. mojavensis lineage, two of them occur in the active site pocket, potentially having a significant effect on substrate specificity and in the adaptation to alternative cactus hosts.
Authors:
Luciano M Matzkin
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2008-02-01
Journal Detail:
Title:  Genetics     Volume:  178     ISSN:  0016-6731     ISO Abbreviation:  Genetics     Publication Date:  2008 Feb 
Date Detail:
Created Date:  2008-02-21     Completed Date:  2008-06-02     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  0374636     Medline TA:  Genetics     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1073-83     Citation Subset:  IM    
Affiliation:
Department of Ecology and Evolutionary Biology and Center for Insect Science, University of Arizona, Tucson, Arizona 85721, USA. lmatzkin@email.arizona.edu
Data Bank Information
Bank Name/Acc. No.:
GENBANK/EU079380;  EU079381;  EU079382;  EU079383;  EU079384;  EU079385;  EU079386;  EU079387;  EU079388;  EU079389;  EU079390;  EU079391;  EU079392;  EU079393;  EU079394;  EU079395;  EU079396;  EU079397;  EU079398;  EU079399;  EU079400;  EU079401;  EU079402;  EU079403;  EU079404;  EU079405;  EU079406;  EU079407;  EU079408;  EU079409;  EU079410;  EU079411;  EU079412;  EU079413;  EU079414;  EU079415;  EU079416;  EU079417;  EU079418;  EU079419;  EU079420;  EU079421;  EU079422;  EU079423;  EU079424;  EU079425;  EU079426;  EU079427;  EU079428;  EU079429;  EU079430;  EU079431;  EU079432;  EU079433;  EU079434;  EU079435;  EU079436;  EU079437;  EU079438;  EU079439;  EU079440;  EU079441;  EU079442;  EU079443;  EU079444;  EU079445;  EU079446;  EU079447;  EU079448;  EU079449;  EU079450;  EU079451;  EU079452;  EU079453;  EU079454;  EU079455;  EU079456;  EU079457;  EU079458;  EU079459;  EU079460;  EU079461;  EU079462;  EU079463;  EU079464;  EU079465;  EU079466;  EU079467;  EU079468;  EU079469;  EU079470;  EU079471
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MeSH Terms
Descriptor/Qualifier:
Acclimatization
Animals
Desert Climate
Drosophila / classification,  enzymology*,  genetics*
Drosophila Proteins / genetics
Evolution, Molecular*
Genetic Variation
Geography
Glutathione Transferase / genetics*
Mexico
Molecular Sequence Data
Phylogeny
United States
Grant Support
ID/Acronym/Agency:
2K12 G000798-06//PHS HHS
Chemical
Reg. No./Substance:
0/Drosophila Proteins; EC 2.5.1.18/Glutathione Transferase
Comments/Corrections

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