Document Detail

A molecular basis of cryopreservation failure and its modulation to improve cell survival.
MedLine Citation:
PMID:  11714190     Owner:  NLM     Status:  MEDLINE    
The requirement for more effective cryopreservation (CP) methodologies in support of the emerging fields of cell bioprocessing and cell therapy is now critical. Current CP strategies appropriately focus on minimizing the damaging actions of physicochemical stressors and membrane disruption associated with extra- and intracellular ice formation that occurs during the freeze-thaw process. CP protocols derived from this conceptual paradigm, however, yield suboptimal survival rates. We now provide the first report on the identification of delayed-onset cell death following CP and the significance of modulating molecular biological aspects of the cellular responses (apoptosis) to low temperature as an essential component to improve postthaw outcome. In this study we quantitatively examined the molecular basis of cell death associated with CP failure in a canine renal cell model. In addition, we report on the significant improvement in CP outcome through the modulation of these molecular mechanisms by the utilization of an organ preservation solution. HypoThermosol. Further, the utilization of HypoThermosol as the preservation medium and the modulation of molecular-based cell death have led to a paradigm shift in biologic preservation methodologies. The recognition of molecular mechanisms associated with CP-induced cell death offers the promise of improved CP of more complex and/or fragile biological systems such as stem cells, engineered tissues, and human organs.
J M Baust; M J Vogel; R Van Buskirk; J G Baust
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Cell transplantation     Volume:  10     ISSN:  0963-6897     ISO Abbreviation:  Cell Transplant     Publication Date:  2001  
Date Detail:
Created Date:  2001-11-20     Completed Date:  2002-04-09     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  9208854     Medline TA:  Cell Transplant     Country:  United States    
Other Details:
Languages:  eng     Pagination:  561-71     Citation Subset:  IM    
Institute of Biomedical Technology, State University of New York, Binghamton 13902, USA.
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MeSH Terms
Annexin A5 / analysis
Apoptosis / drug effects
Caspase 3
Caspases / metabolism
Cell Line / cytology,  transplantation
Cell Survival / drug effects
Cell Transplantation / methods*
Cryopreservation / methods*
Cryoprotective Agents / pharmacology*
Culture Media / pharmacology
Endopeptidases / metabolism
Extracellular Space
Kidney / cytology
Grant Support
1 R43 RR14185-01/RR/NCRR NIH HHS
Reg. No./Substance:
0/Annexin A5; 0/Cryoprotective Agents; 0/Culture Media; EC 3.4.-/Endopeptidases; EC 3.4.22.-/CASP3 protein, human; EC 3.4.22.-/Caspase 3; EC 3.4.22.-/Caspases

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