| The molar extinction of rhodopsin. | |
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MedLine Citation:
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PMID: 13109155 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The molar extinction of rhodopsin is 40,600 cm.(2) per mole equivalent of retinene; i.e., this is the extinction of a solution of rhodopsin which is produced by, or yields on bleaching, a molar solution of retinene. The molar extinctions of all-trans retinene and all-trans retinene oxime have also been determined in ethyl alcohol and aqueous digitonin solutions. On the assumption that each chromophoric group of rhodopsin is made from a single molecule of retinene, it is concluded that the primary photochemical conversion of rhodopsin to lumi-rhodopsin has a quantum efficiency of 1; though the over-all bleaching of rhodopsin in solution to retinene and opsin may have a quantum efficiency as low as one-half. On bleaching cattle rhodopsin, about two sulfhydryl groups appear for each molecule of retinene liberated. In frog rhodopsin the -SH:retinene ratio appears to be higher, 5:2 or perhaps even 3:1. Some of this sulfhydryl appears to have been engaged in binding retinene to opsin; some may have been exposed as the result of changes in opsin which accompany bleaching, comparable with protein denaturation. |
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Authors:
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G WALD; P K BROWN |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: The Journal of general physiology Volume: 37 ISSN: 0022-1295 ISO Abbreviation: J. Gen. Physiol. Publication Date: 1953 Nov |
Date Detail:
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Created Date: 1954-12-01 Completed Date: 2003-05-01 Revised Date: 2010-06-22 |
Medline Journal Info:
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Nlm Unique ID: 2985110R Medline TA: J Gen Physiol Country: Not Available |
Other Details:
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Languages: eng Pagination: 189-200 Citation Subset: OM |
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| MeSH Terms | |
Descriptor/Qualifier:
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Rhodopsin* |
| Chemical | |
Reg. No./Substance:
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9009-81-8/Rhodopsin |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
| Full Text | |
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Journal Information Journal ID (nlm-ta): J Gen Physiol ISSN: 0022-1295 ISSN: 1540-7748 Publisher: The Rockefeller University Press |
Article Information Download PDF  Copyright © Copyright, 1953, by The Rockefeller Institute for Medical Research Received Day: 11 Month: 6 Year: 1953 Print publication date: Day: 20 Month: 11 Year: 1953 Volume: 37 Issue: 2 First Page: 189 Last Page: 200 ID: 2147432 PubMed Id: 13109155 |
| THE MOLAR EXTINCTION OF RHODOPSIN | |
| George Wald | |
| Paul K. Brown | |
| From the Biological Laboratories of Harvard University, Cambridge |
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