Document Detail


A modifier screen in the eye reveals control genes for Krüppel activity in the Drosophila embryo.
MedLine Citation:
PMID:  9724781     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Irregular facets (If) is a dominant mutation of Drosophila that results in small eyes with fused ommatidia. Previous results showed that the gene Krüppel (Kr), which is best known for its early segmentation function, is expressed ectopically in If mutant eye discs. However, it was not known whether ectopic Kr activity is either the cause or the result of the If mutation. Here, we show that If is a gain-of-function allele of Kr. We then used the If mutation in a genetic screen to identify dominant enhancers and suppressors of Kr activity on the third chromosome. Of 30 identified Kr-interacting loci, two were cloned, and we examined whether they also represent components of a natural Kr-dependent developmental pathway of the embryo. We show that the two genes, eyelid (eld) and extramacrochaetae (emc), which encode a Bright family-type DNA binding protein and a helix-loop-helix factor, respectively, are necessary to achieve the singling-out of a unique Kr-expressing cell during the development of the Malpighian tubules, the excretory organs of the fly. The results indicate that the Kr gain-of-function mutation If provides a tool to identify genes that are active during eye development and that a number of them function also in the control of Kr-dependent developmental processes.
Authors:
P Carrera; S Abrell; B Kerber; U Walldorf; A Preiss; M Hoch; H Jäckle
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Proceedings of the National Academy of Sciences of the United States of America     Volume:  95     ISSN:  0027-8424     ISO Abbreviation:  Proc. Natl. Acad. Sci. U.S.A.     Publication Date:  1998 Sep 
Date Detail:
Created Date:  1998-09-28     Completed Date:  1998-09-28     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  7505876     Medline TA:  Proc Natl Acad Sci U S A     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  10779-84     Citation Subset:  IM    
Affiliation:
Abteilung Molekulare Entwicklungsbiologie, Max-Planck-Institut für biophysikalische Chemie, Am Fassberg, D-37077 Göttingen, Germany.
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MeSH Terms
Descriptor/Qualifier:
Amino Acid Sequence
Animals
Base Sequence
Cell Division
DNA-Binding Proteins / genetics*
Drosophila / embryology,  genetics*
Drosophila Proteins
Embryo, Nonmammalian
Eye / embryology,  metabolism*,  ultrastructure
Gene Expression Regulation, Developmental
Kruppel-Like Transcription Factors
Microscopy, Electron, Scanning
Mutagenesis
Oligodeoxyribonucleotides
Repressor Proteins*
Transcription Factors / genetics*
Zinc Fingers / genetics*
Chemical
Reg. No./Substance:
0/DNA-Binding Proteins; 0/Drosophila Proteins; 0/Kruppel protein, Drosophila; 0/Kruppel-Like Transcription Factors; 0/Oligodeoxyribonucleotides; 0/Repressor Proteins; 0/Transcription Factors
Comments/Corrections

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