| A modified tandem affinity purification tag technique for the purification of protein complexes in mammalian cells. | |
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MedLine Citation:
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PMID: 15602769 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The tandem affinity purification (TAP) tag technique has been used with success to identify under nondenaturing conditions protein complexes in yeast. The technique can be used in mammalian cells, but we found that the original technique does not yield enough recovery for the identification of proteins when mammalian cells growing in monolayer have to be used. We present here a modified TAP tag technique that allows sufficient recovery of proteins from mouse fibroblasts growing in monolayer cultures. The recovery allows protein identification by mass spectrometry. |
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Authors:
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Robert Drakas; Marco Prisco; Renato Baserga |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Proteomics Volume: 5 ISSN: 1615-9853 ISO Abbreviation: Proteomics Publication Date: 2005 Jan |
Date Detail:
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Created Date: 2005-02-24 Completed Date: 2005-08-08 Revised Date: 2008-11-21 |
Medline Journal Info:
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Nlm Unique ID: 101092707 Medline TA: Proteomics Country: Germany |
Other Details:
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Languages: eng Pagination: 132-7 Citation Subset: IM |
Affiliation:
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Kimmel Cancer Center, Thomas Jefferson University, Philadelphia, PA 19107, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Affinity Labels
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chemistry* Animals Biotinylation Cell Line Cell Proliferation Embryo, Mammalian / cytology Escherichia coli Proteins / genetics Fibroblasts / chemistry, cytology Humans Immunoprecipitation Insulin Receptor Substrate Proteins Insulin-Like Growth Factor I / pharmacology Mass Spectrometry Mice Multiprotein Complexes / chemistry, genetics, isolation & purification* Phosphoproteins / chemistry, genetics, isolation & purification* Plasmids Recombinant Fusion Proteins / chemistry, genetics, isolation & purification |
| Grant Support | |
ID/Acronym/Agency:
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AG20956/AG/NIA NIH HHS; CA86940/CA/NCI NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Affinity Labels; 0/Escherichia coli Proteins; 0/IRS1 protein, human; 0/Insulin Receptor Substrate Proteins; 0/Irs1 protein, mouse; 0/Multiprotein Complexes; 0/Phosphoproteins; 0/Recombinant Fusion Proteins; 67763-96-6/Insulin-Like Growth Factor I |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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