Document Detail


A modified system for thiazolinone conversion to thiohydantoin derivatives and their separation by high-pressure liquid chromatography.
MedLine Citation:
PMID:  4091269     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
An improved and very simple procedure for thiazolinone conversion to thiohydantoin derivatives and their separation by reverse-phase high-pressure liquid chromatography is described. Trifluoroacetic acid (10%) in ethyl acetate has been employed as a conversion reagent to circumvent the deamidation of acid amides and methylation of acidic amino acids, with a concomitant increase in the detection limits of these residues. Additionally, a very simple procedure has been developed for the separation of phenylthiohydantoin (PTH) derivatives of amino acids. The system takes advantage of the computer-controlled precise mixing of the solvents A and B to achieve accurate pH and thus avoid the necessity of pH adjustment of a buffer. The procedure is simple and highly reproducible, and separates all the 20 known PTH amino acids. The efficiency of the method has been examined on synthetic and natural proteins/peptides, in manual and autoconversion systems, over a period of more than 18 months.
Authors:
A S Bhown; J C Bennett
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Analytical biochemistry     Volume:  150     ISSN:  0003-2697     ISO Abbreviation:  Anal. Biochem.     Publication Date:  1985 Nov 
Date Detail:
Created Date:  1986-02-24     Completed Date:  1986-02-24     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  0370535     Medline TA:  Anal Biochem     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  457-62     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Amino Acid Sequence
Amino Acids / analysis
Chemical Phenomena
Chemistry
Chromatography, High Pressure Liquid
Hydantoins / isolation & purification*
Hydrogen-Ion Concentration
Solvents
Thiazoles*
Thiohydantoins / isolation & purification*
Grant Support
ID/Acronym/Agency:
AM-03555/AM/NIADDK NIH HHS; AM-20614/AM/NIADDK NIH HHS
Chemical
Reg. No./Substance:
0/Amino Acids; 0/Hydantoins; 0/Solvents; 0/Thiazoles; 0/Thiohydantoins

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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