Document Detail


A modified rapid enzymatic microtiter plate assay for the quantification of intracellular γ-aminobutyric acid and succinate semialdehyde in bacterial cells.
MedLine Citation:
PMID:  21044648     Owner:  NLM     Status:  In-Process    
Abstract/OtherAbstract:
The GABase assay is widely used to rapidly and accurately quantify levels of extracellular γ-aminobutyric acid (GABA). Here we demonstrate a modification of this assay that enables quantification of intracellular GABA in bacterial cells. Cells are lysed by boiling and ethanolamine-O-sulphate, a GABA transaminase inhibitor is used to distinguish between GABA and succinate semialdehyde.
Authors:
C P O'Byrne; C Feehily; R Ham; K A G Karatzas
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2010-10-31
Journal Detail:
Title:  Journal of microbiological methods     Volume:  84     ISSN:  1872-8359     ISO Abbreviation:  J. Microbiol. Methods     Publication Date:  2011 Jan 
Date Detail:
Created Date:  2010-12-20     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8306883     Medline TA:  J Microbiol Methods     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  137-9     Citation Subset:  IM    
Copyright Information:
Copyright © 2010 Elsevier B.V. All rights reserved.
Affiliation:
Bacterial Stress Response Group, Microbiology, School of Natural Sciences, NUI Galway, Galway, Ireland.
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