| A modified rapid enzymatic microtiter plate assay for the quantification of intracellular γ-aminobutyric acid and succinate semialdehyde in bacterial cells. | |
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MedLine Citation:
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PMID: 21044648 Owner: NLM Status: In-Process |
Abstract/OtherAbstract:
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The GABase assay is widely used to rapidly and accurately quantify levels of extracellular γ-aminobutyric acid (GABA). Here we demonstrate a modification of this assay that enables quantification of intracellular GABA in bacterial cells. Cells are lysed by boiling and ethanolamine-O-sulphate, a GABA transaminase inhibitor is used to distinguish between GABA and succinate semialdehyde. |
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Authors:
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C P O'Byrne; C Feehily; R Ham; K A G Karatzas |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2010-10-31 |
Journal Detail:
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Title: Journal of microbiological methods Volume: 84 ISSN: 1872-8359 ISO Abbreviation: J. Microbiol. Methods Publication Date: 2011 Jan |
Date Detail:
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Created Date: 2010-12-20 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8306883 Medline TA: J Microbiol Methods Country: Netherlands |
Other Details:
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Languages: eng Pagination: 137-9 Citation Subset: IM |
Copyright Information:
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Copyright © 2010 Elsevier B.V. All rights reserved. |
Affiliation:
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Bacterial Stress Response Group, Microbiology, School of Natural Sciences, NUI Galway, Galway, Ireland. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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