Document Detail

A modified method for measurement of radiotracer permeation across the rat blood--brain barrier: the problem of correcting brain uptake for intravascular tracer.
MedLine Citation:
PMID:  6415348     Owner:  NLM     Status:  MEDLINE    
A modified technique to assess integrity of the rat blood--brain barrier is described in which a poorly permeating radiotracer is injected intravenously and its net permeation across the cerebral vasculature in 30 min is related to its time-averaged level in the circulation. At the termination of experiments, direct carotid arterial perfusion clears blood from brain in 30 s and permits measurement of the tracer level in brain parenchyma without correction for intravascular tracer. Constant-rate withdrawal of a single arterial blood sample throughout the period of tracer circulation replaces the need for repeated blood sampling and graph plotting to determine the time-integrated plasma tracer level. During the first 2-3 min following intravenous injection of [3H]mannitol or [14C]sucrose, permeation across the barrier occurred at a rate substantially greater than that measured for a 30 min circulation time. The relatively rapid uptake of these tracers into brain during the first few minutes after injection would explain in part why estimates of regional blood volume, calculated from ratios of brain vs blood tracer concentration after short circulation times, were much higher than values determined using [51Cr]erythrocytes.
E Preston; M Allen; N Haas
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of neuroscience methods     Volume:  9     ISSN:  0165-0270     ISO Abbreviation:  J. Neurosci. Methods     Publication Date:  1983 Sep 
Date Detail:
Created Date:  1983-12-20     Completed Date:  1983-12-20     Revised Date:  2003-11-14    
Medline Journal Info:
Nlm Unique ID:  7905558     Medline TA:  J Neurosci Methods     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  45-55     Citation Subset:  IM    
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MeSH Terms
Blood Volume
Blood-Brain Barrier*
Brain / metabolism
Brain Mapping / methods*
Carbon Radioisotopes / metabolism*
Mannitol / metabolism*
Rats, Inbred Strains
Regional Blood Flow
Sucrose / metabolism*
Tritium / metabolism*
Reg. No./Substance:
0/Carbon Radioisotopes; 10028-17-8/Tritium; 57-50-1/Sucrose; 69-65-8/Mannitol

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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