Document Detail


A modified electrode for the electrochemical detection of biogenic amines and their amino acid precursors separated by microchip capillary electrophoresis.
MedLine Citation:
PMID:  21437919     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
The use of a mixed-valent ruthenium oxide/hexacyanoruthenate polymeric film electrochemically deposited onto glassy carbon electrodes is proposed here for the detection of biogenic amines and their amino acid precursors, following their separation by microchip capillary electrophoresis. The ability of this ruthenium coating to electrocatalyze the oxidation of aliphatic and heterocyclic amines, as well as their amino acid precursors, was checked by using ethanolamine, tryptamine and tryptophane as prototype compounds and adopting a 25 mM sulphuric acid as the electrolyte in the detection cell, where a constant potential of 1.05 V versus Ag/AgCl, 3 M KCl was applied to the modified working electrode. Optimization of parameters affecting both detection and separation steps led to satisfactory separations when performed by using a 20 mM phosphate running buffer (pH 2.5) and applying a high voltage of 2.5 kV both in the separation and in the electrokinetic injection (duration 4 s). The recorded peaks were characterized by good repeatability (RSD≤3.6%), high sensitivity and a wide linear range. Detection limits of 23 μM (1.4 mg/L), 27 μM (4.3 mg/L) and 34 μM (6.8 mg/L) were inferred for ethanolamine, tryptamine and tryptophane, respectively. The approach proposed here was also applied for the analysis of some double malt dark beers spiked with a controlled amount of the analytes considered.
Authors:
Nicolò Dossi; Rosanna Toniolo; Andrea Pizzariello; Sabina Susmel; Gino Bontempelli
Related Documents :
21591669 - Lead detoxification activities of a class of novel dmsa-amino acid conjugates.
21380759 - Perinodal adipose tissue and mesenteric lymph node activation during reactivated tnbs-c...
8371069 - Collision-induced dissociation of f2-isoprostane-containing phospholipids.
18314329 - Antioxidant capacity of bo-653, 2,3-dihydro-5-hydroxy-4,6-di-tert-butyl-2,2-dipentylben...
11698839 - An in vivo comparison of topical agents on wound repair.
21158399 - Aggregation and ph responsive disassembly of a new acid-labile surfactant synthesized b...
10380169 - Adaptive and non-adaptive responses in rats exposed to ozone, alone and in mixtures, wi...
6139419 - Changes in the amino acid content of nerve endings (synaptosomes) induced by drugs that...
19118369 - Mechanism of conjugated linoleic acid and vaccenic acid formation in human faecal suspe...
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2011-3-16
Journal Detail:
Title:  Electrophoresis     Volume:  -     ISSN:  1522-2683     ISO Abbreviation:  -     Publication Date:  2011 Mar 
Date Detail:
Created Date:  2011-3-25     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8204476     Medline TA:  Electrophoresis     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © 2011 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
Affiliation:
Department of Chemical Science and Technology, University of Udine, Udine, Italy.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  In-channel amperometric detection for microchip electrophoresis using a wireless isolated potentiost...
Next Document:  Lymphoma development of simultaneously combined exposure to two radiofrequency signals in AKR/J mice...