Document Detail


A modified differential scanning calorimetry for determination of cell volumetric change during the freezing process.
MedLine Citation:
PMID:  12391483     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A modified analytical and experimental method using differential scanning calorimeter (DSC) was developed to determine the cell volume change during the freezing process. Two cell types were used in the study: human platelets and erythrocytes (red blood cells). Isotonic cell suspensions with different cytocrits were prepared and used in the DSC experiments. Low cooling rates were used to avoid intracellular ice formation. Cell suspensions were cooled from room temperature to -40 degrees C. Latent heat release from the freezing of cell suspensions was shown to be a linear function of cytocrit. From slope and intercept of the linear function, cell volume change was determined based on a developed theoretical model. From experimental data and theoretical analyses, it was revealed that (a) the final volume of a human platelet at -40 degrees C was 33.7% of its isotonic volume, and 15.2% of the original (at isotonic condition) intracellular water remained unfrozen inside platelets, and (b) the final volume of human erythrocyte at -40 degrees C was 50.0% of its isotonic volume, and 30.3% of the original intracellular water was kept inside cells as residual unfrozen water.
Authors:
Dawei Luo; Xu Han; Liqun He; Xiangdong Cui; Shuxia Cheng; Caicheng Lu; Jianghan Liu; Dayong Gao
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.; Validation Studies    
Journal Detail:
Title:  Cryo letters     Volume:  23     ISSN:  0143-2044     ISO Abbreviation:  Cryo Letters     Publication Date:    2002 Jul-Aug
Date Detail:
Created Date:  2002-10-22     Completed Date:  2002-12-09     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  9891832     Medline TA:  Cryo Letters     Country:  England    
Other Details:
Languages:  eng     Pagination:  229-36     Citation Subset:  IM    
Affiliation:
Department of Mechanical Engineering and Center for Biomedical Engineering, University of Kentucky, Lexington, KY 40506, USA.
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MeSH Terms
Descriptor/Qualifier:
Blood Platelets / cytology*
Calorimetry, Differential Scanning / standards*
Cell Size
Cryopreservation*
Erythrocytes / cytology*
Humans
Reproducibility of Results
Grant Support
ID/Acronym/Agency:
RG990038/RG/CSR NIH HHS

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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