| A modified apparatus for dual, sterilized, isolated perfusion of the rat liver. | |
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MedLine Citation:
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PMID: 2291894 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The isolated perfused rat liver (IPRL) has proven to be a useful model for the study of physiology and pathology of the liver. For research in nonparenchymal cell (NPC) function that includes measurement of cytokine production (eg, TNF), it is necessary to have a sterilized perfusion system. We have modified the IPRL apparatus so as to be able to perform sterile perfusions of two livers simultaneously. The perfusion apparatus is a recirculating closed system in which the oxygenator is a plastic container separated into two chambers by a fenestrated plastic wall. A disposable macropore filter functions as both a bubble trap and perfusate filter. The sterilization process is done by immersing the various components in Benz-All solution. The tubing is disinfected by irrigation with 10% Clorox followed by 0.9% sodium chloride solution. The perfusate used is filter-sterilized Krebs buffer solution containing 0.5 g Mandol/250 mL perfusate. Not only can two organs be conveniently perfused simultaneously, but the entire system can be reliably sterilized for up to 20 consecutive perfusions. Bile production is higher and more stable with less leakage of intracellular enzymes. Many of the components are disposable and can be altered to suit the needs of a particular experiment. |
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Authors:
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T Liu; T R Walsh; H Mischinger; P N Rao; P Chelvakumar; R Rubin; T E Starzl |
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Publication Detail:
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Type: Comparative Study; In Vitro; Journal Article; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Journal of investigative surgery : the official journal of the Academy of Surgical Research Volume: 3 ISSN: 0894-1939 ISO Abbreviation: J Invest Surg Publication Date: 1990 |
Date Detail:
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Created Date: 1991-04-09 Completed Date: 1991-04-09 Revised Date: 2012-05-07 |
Medline Journal Info:
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Nlm Unique ID: 8809255 Medline TA: J Invest Surg Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 365-72 Citation Subset: IM |
Affiliation:
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Department of Surgery, University of Pittsburgh, PA 15261. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Bile / metabolism Liver / enzymology, metabolism* Male Perfusion / instrumentation* Rats Rats, Inbred Lew Sterilization Tumor Necrosis Factor-alpha / biosynthesis |
| Grant Support | |
ID/Acronym/Agency:
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DK29961/DK/NIDDK NIH HHS; R01 DK029961-19/DK/NIDDK NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Tumor Necrosis Factor-alpha |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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