| The modification of the membrane of Oceanomonas baumannii when subjected to both osmotic and organic solvent stress. | |
| | |
MedLine Citation:
|
PMID: 10930729 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Oceanomonas baumanniioff a novel halotolerant bacterium which was isolated from the estuary of the river Wear (Sunderland, UK). When grown in tryptone soya broth it can tolerate high levels of phenol, which is not utilised as a carbon source in this medium. However, the level of tolerance was reduced from 35 mM to 3 mM phenol as salinity increased from 1% to 12% NaCl (w/v). Increasing salinity up to 12% NaCl also decreased the growth rate 8-fold and caused modifications to the cytoplasmic membrane particularly anionic phosphatidylglycerol levels, which doubled at the expense of zwitterionic phosphatidylethanolamine. In addition, changes in the phospholipid fatty acid composition were noted, cis-vaccenic acid decreased significantly at higher salinities. Intracellular solute levels also increased with increasing salinity and there was an accumulation of the compatible solutes ectoine, glycine betaine and glutamate. The addition of phenol to osmotically compromised cultures led to a further modification of the cytoplasmic membrane phospholipid composition, in particular, that the decrease in zwitterionic phosphatidylethanolamine and the increase of anionic phospholipid species was much less pronounced. A further decrease in unsaturation, particularly in the proportion of cis-vaccenic acid, and the mean chain length of the fatty acids suggested that this response was important in maintaining membrane integrity in the presence of phenol. |
| | |
Authors:
|
G R Brown; I C Sutcliffe; D Bendell; S P Cummings |
Related Documents
:
|
8068729 - Dietary fat alters membrane composition in rod outer segments in normal and diabetic ra... 11724659 - Effects of phytanic acid on the vitamin e status, lipid composition and physical proper... 17096349 - Familial adenomatous polyposis patients have high levels of arachidonic acid and docosa... 3965219 - Variance estimates and individuality ratios of 25 serum constituents in beagles. 2958089 - Labeling of lipids of retina subcellular fractions by [1-14c]eicosatetraenoate (20:4(n-... 6302539 - Comparison of membrane structure, osmotic fragility, and morphology of multiple scleros... 8105749 - Replacement of margarine on bread by rapeseed and olive oils: effects on plasma fatty a... 1775479 - Sugar, fluoride, ph and microbial homeostasis in dental plaque. 15731499 - The ileocyte basolateral organic solute transporter (ostalpha-ostbeta) complex: finding... |
Publication Detail:
|
Type: Journal Article |
Journal Detail:
|
Title: FEMS microbiology letters Volume: 189 ISSN: 0378-1097 ISO Abbreviation: FEMS Microbiol. Lett. Publication Date: 2000 Aug |
Date Detail:
|
Created Date: 2000-09-12 Completed Date: 2000-09-12 Revised Date: 2000-12-18 |
Medline Journal Info:
|
Nlm Unique ID: 7705721 Medline TA: FEMS Microbiol Lett Country: NETHERLANDS |
Other Details:
|
Languages: eng Pagination: 149-54 Citation Subset: IM |
Affiliation:
|
The School of Sciences, University of Sunderland, SR1 3SD, Sunderland, UK. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Cell Membrane
/
drug effects*,
physiology Gram-Negative Bacteria / drug effects*, physiology*, ultrastructure Osmotic Pressure Phenols / toxicity* Solvents / toxicity* |
| Chemical | |
Reg. No./Substance:
|
0/Phenols; 0/Solvents |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Isolation and characterization of acid- and Al-tolerant microorganisms.
Next Document: Immuno-crossreactivity and toxicity assessment of conjugation products of the cyanobacterial toxin, ...