| The modification of induced genetic change in yeast by an amino acid analogue. | |
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MedLine Citation:
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PMID: 353529 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Treatment of diploid yeast cultures with the amino acid analogue, para-fluorophenylalanine (PFPA), at concentrations which caused inhibition of growth, resulted in up to 5 fold increases in the frequency of mitotic gene conversion at two different heteroallelic loci. With haploid yeast cultures, growth in PFPA increased the rate of forward mutation to canavanine resistance by at least 2 fold. Growth of diploids in PFPA prior to exposure to the deaminating agent nitrous acid, the cross-linking agent mitomycin C, the alkylating chemical ethylmethanesulphonate (EMS) and UV light resulted in significant changes in the potency of these diverse mutagens to induce intragenic recombination. For all four mutagens, increased frequencies of gene convertants/viable cell were observed in those cultures which had been exposed to the amino acid analogue prior to mutagen treatment. In haploid WT yeast cells, amino acid analogue incorporation resulted in an enhanced frequency of UV induced forward mutation to canavanine resistance whilst in a DNA repair deficient rad 6 mutant this interaction between UV and PFPA was abolished. The results have been interpreted on the basis of incorporation of the analogue into enzymes involved with DNA replication with a consequent loss of fidelity of such enzymes and increased errors in base incorporation. |
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Authors:
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P J Davies; J M Parry |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Molecular & general genetics : MGG Volume: 162 ISSN: 0026-8925 ISO Abbreviation: Mol. Gen. Genet. Publication Date: 1978 Jun |
Date Detail:
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Created Date: 1978-09-15 Completed Date: 1978-09-15 Revised Date: 2000-12-18 |
Medline Journal Info:
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Nlm Unique ID: 0125036 Medline TA: Mol Gen Genet Country: GERMANY, WEST |
Other Details:
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Languages: eng Pagination: 183-90 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Alleles* DNA Repair DNA Replication* Mutagens Phenylalanine / analogs & derivatives* Recombination, Genetic / drug effects Saccharomyces cerevisiae / genetics, radiation effects Ultraviolet Rays p-Fluorophenylalanine / pharmacology* |
| Chemical | |
Reg. No./Substance:
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0/Mutagens; 60-17-3/p-Fluorophenylalanine; 63-91-2/Phenylalanine |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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