Document Detail

The modification of induced genetic change in yeast by an amino acid analogue.
MedLine Citation:
PMID:  353529     Owner:  NLM     Status:  MEDLINE    
Treatment of diploid yeast cultures with the amino acid analogue, para-fluorophenylalanine (PFPA), at concentrations which caused inhibition of growth, resulted in up to 5 fold increases in the frequency of mitotic gene conversion at two different heteroallelic loci. With haploid yeast cultures, growth in PFPA increased the rate of forward mutation to canavanine resistance by at least 2 fold. Growth of diploids in PFPA prior to exposure to the deaminating agent nitrous acid, the cross-linking agent mitomycin C, the alkylating chemical ethylmethanesulphonate (EMS) and UV light resulted in significant changes in the potency of these diverse mutagens to induce intragenic recombination. For all four mutagens, increased frequencies of gene convertants/viable cell were observed in those cultures which had been exposed to the amino acid analogue prior to mutagen treatment. In haploid WT yeast cells, amino acid analogue incorporation resulted in an enhanced frequency of UV induced forward mutation to canavanine resistance whilst in a DNA repair deficient rad 6 mutant this interaction between UV and PFPA was abolished. The results have been interpreted on the basis of incorporation of the analogue into enzymes involved with DNA replication with a consequent loss of fidelity of such enzymes and increased errors in base incorporation.
P J Davies; J M Parry
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Molecular & general genetics : MGG     Volume:  162     ISSN:  0026-8925     ISO Abbreviation:  Mol. Gen. Genet.     Publication Date:  1978 Jun 
Date Detail:
Created Date:  1978-09-15     Completed Date:  1978-09-15     Revised Date:  2000-12-18    
Medline Journal Info:
Nlm Unique ID:  0125036     Medline TA:  Mol Gen Genet     Country:  GERMANY, WEST    
Other Details:
Languages:  eng     Pagination:  183-90     Citation Subset:  IM    
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MeSH Terms
DNA Repair
DNA Replication*
Phenylalanine / analogs & derivatives*
Recombination, Genetic / drug effects
Saccharomyces cerevisiae / genetics,  radiation effects
Ultraviolet Rays
p-Fluorophenylalanine / pharmacology*
Reg. No./Substance:
0/Mutagens; 60-17-3/p-Fluorophenylalanine; 63-91-2/Phenylalanine

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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